30730

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2207156..2208024 Replicon chromosome
Accession NZ_AP014573
Organism Mycobacterium tuberculosis str. Kurono

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag KURONO_RS10285 Protein ID WP_010886136.1
Coordinates 2207156..2207533 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag KURONO_RS10290 Protein ID WP_003409886.1
Coordinates 2207575..2208024 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KURONO_RS10235 2202945..2203397 - 453 WP_003899095.1 lipoprotein -
KURONO_RS10240 2203461..2203862 + 402 WP_003409869.1 hypothetical protein -
KURONO_RS10245 2203855..2204037 - 183 WP_003409870.1 hypothetical protein -
KURONO_RS10250 2204151..2204501 - 351 WP_003899096.1 hypothetical protein -
KURONO_RS10255 2204512..2205414 - 903 WP_003899097.1 hypothetical protein -
KURONO_RS10260 2205435..2205626 - 192 WP_003409876.1 hypothetical protein -
KURONO_RS10265 2205627..2205923 - 297 WP_003409877.1 hypothetical protein -
KURONO_RS10270 2206163..2206378 + 216 WP_003409878.1 antitoxin -
KURONO_RS10275 2206375..2206686 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
KURONO_RS22510 2206660..2207181 - 522 WP_003904745.1 hypothetical protein -
KURONO_RS10285 2207156..2207533 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
KURONO_RS10290 2207575..2208024 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KURONO_RS10295 2208021..2208566 + 546 WP_003409891.1 SecB-like chaperone -
KURONO_RS10300 2208455..2209069 - 615 WP_003901296.1 hypothetical protein -
KURONO_RS10305 2209118..2209414 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
KURONO_RS10310 2209411..2209662 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
KURONO_RS10315 2209649..2210143 + 495 WP_003899099.1 hypothetical protein -
KURONO_RS10320 2210303..2210710 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
KURONO_RS10325 2210714..2210986 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
KURONO_RS10330 2211019..2212239 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T30730 WP_010886136.1 NZ_AP014573:2207156-2207533 [Mycobacterium tuberculosis str. Kurono]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T30730 NZ_AP014573:2207156-2207533 [Mycobacterium tuberculosis str. Kurono]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT30730 WP_003409886.1 NZ_AP014573:2207575-2208024 [Mycobacterium tuberculosis str. Kurono]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT30730 NZ_AP014573:2207575-2208024 [Mycobacterium tuberculosis str. Kurono]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
AlphaFold DB G0TLM0

References