Detailed information of TA system TA030689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 702132..702970
• Replicon: chromosome
• Accession: NZ_AP014569
• Organism: Serpentinomonas mccroryi strain B1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A060NKC2
• Locus tag: SMCB_RS03255
• Protein ID: WP_045535025.1
• Coordinates: 702458..702970 (+)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A060NNG3
• Locus tag: SMCB_RS03250
• Protein ID: WP_045535023.1
• Coordinates: 702132..702461 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMCB_RS03235	697979..699535	-	1557	WP_045535020.1	SAVED domain-containing protein	-
SMCB_RS12395	699749..700714	+	966	Protein_650	IS481 family transposase	-
SMCB_RS03245	700722..701612	-	891	Protein_651	integrase arm-type DNA-binding domain-containing protein	-
SMCB_RS03250	702132..702461	+	330	WP_045535023.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
SMCB_RS03255	702458..702970	+	513	WP_045535025.1	type II toxin-antitoxin system YhaV family toxin	Toxin
SMCB_RS03260	702970..703161	+	192	WP_045535027.1	DUF1778 domain-containing protein	-
SMCB_RS12665	703218..703625	+	408	Protein_655	helix-turn-helix domain-containing protein	-
SMCB_RS03270	703945..704193	-	249	WP_045535029.1	hypothetical protein	-
SMCB_RS03275	704210..705418	-	1209	WP_045535031.1	XRE family transcriptional regulator	-
SMCB_RS03280	705415..706071	-	657	WP_045537530.1	hypothetical protein	-
SMCB_RS03285	706232..707557	-	1326	WP_045535033.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	699749..700951	1202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-153)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 19660.48 Da
• Isoelectric Point: 9.4943

>T30689 WP_045535025.1 NZ_AP014569:702458-702970 [Serpentinomonas mccroryi]
MSSNKPAPLLIHDWTVFAHPLFLAQIEVIVQQVEVLKERDPVGYVKKNATKRLAAIAKLAFDVIPQDPTRPEYRQGNTLG
DDYRHWFRAKFFQQYRLFFRYHAPSKVIVLAWVNDEDTKRAYESGDDAYRVFRKMLASGHPPDDWDQLLKEAKPAADRLQ
SSMGSLKGLE

Nucleotide

• Length: 513 bp

>T30689 NZ_AP014569:702458-702970 [Serpentinomonas mccroryi]
ATGAGCAGCAACAAGCCCGCGCCCCTGCTCATCCACGACTGGACTGTTTTCGCGCACCCGCTGTTTCTCGCGCAAATTGA
GGTCATCGTCCAGCAGGTCGAGGTACTGAAGGAGAGAGACCCTGTCGGATATGTAAAGAAGAACGCCACCAAGAGGCTGG
CGGCAATCGCCAAACTGGCATTCGATGTCATCCCGCAAGACCCGACGAGACCGGAGTACCGACAAGGCAATACGCTCGGC
GACGACTACAGGCACTGGTTCCGGGCCAAGTTTTTCCAGCAGTACCGCTTGTTCTTTCGCTACCACGCACCGAGCAAGGT
CATCGTGCTGGCGTGGGTAAACGATGAGGATACGAAACGCGCCTATGAAAGCGGGGACGATGCATACCGGGTGTTTCGGA
AAATGCTTGCCAGCGGTCACCCGCCCGACGATTGGGATCAGCTACTGAAAGAGGCCAAACCTGCTGCCGACCGACTGCAG
TCCAGCATGGGTTCACTGAAGGGGTTGGAGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12133.66 Da
• Isoelectric Point: 4.4596

>AT30689 WP_045535023.1 NZ_AP014569:702132-702461 [Serpentinomonas mccroryi]
MPATLEVESTLTDRYQTTVPETVRRALRLGKRDKIHYTIRPGGEVVLTRVEAPEVDDPVLGQFLGFLARDITSHPERLQA
IDSTFVQRIQSLTGGIEIDIDATLSADDE

Nucleotide

• Length: 330 bp

>AT30689 NZ_AP014569:702132-702461 [Serpentinomonas mccroryi]
ATGCCTGCCACCCTCGAAGTCGAATCCACGCTGACCGACCGCTACCAGACCACGGTGCCGGAGACGGTGCGTCGCGCCCT
TCGACTCGGTAAGCGCGACAAGATCCACTACACGATCCGCCCCGGCGGCGAGGTCGTGCTGACGCGCGTCGAGGCCCCCG
AAGTTGACGATCCGGTGCTCGGTCAGTTCTTGGGCTTCCTGGCGCGCGACATCACCAGCCATCCGGAGCGCCTGCAGGCC
ATCGACTCCACCTTCGTGCAGCGCATCCAGTCGTTGACCGGCGGCATCGAAATCGACATCGACGCCACCCTGTCGGCGGA
CGATGAATGA

Structures

Toxin

• AlphaFold DB: A0A060NKC2

Antitoxin

• AlphaFold DB: A0A060NNG3