Detailed information of TA system TA030663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 384776..385391
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: SRAA_RS01840
• Protein ID: WP_197538516.1
• Coordinates: 385059..385391 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SRAA_RS01835
• Protein ID: WP_045530658.1
• Coordinates: 384776..385066 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS01810	381476..381769	-	294	WP_045530648.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS01815	381766..381990	-	225	WP_045530650.1	addiction module protein	-
SRAA_RS01820	382073..382762	-	690	WP_045530652.1	hypothetical protein	-
SRAA_RS01825	382996..384087	+	1092	WP_197538515.1	NDP-sugar synthase	-
SRAA_RS01830	384115..384714	+	600	WP_045530656.1	lysophospholipid acyltransferase family protein	-
SRAA_RS01835	384776..385066	-	291	WP_045530658.1	helix-turn-helix domain-containing protein	Antitoxin
SRAA_RS01840	385059..385391	-	333	WP_197538516.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SRAA_RS01845	385520..385744	+	225	WP_082039846.1	hypothetical protein	-
SRAA_RS01850	385856..386176	+	321	WP_045530660.1	transcriptional regulator	-
SRAA_RS12570	386321..386986	-	666	WP_171820201.1	hypothetical protein	-
SRAA_RS01865	387831..389063	+	1233	WP_045530664.1	IS110 family transposase	-
SRAA_RS01870	389274..389606	-	333	WP_045533080.1	HigA family addiction module antidote protein	-
SRAA_RS12575	389621..389704	-	84	WP_082040069.1	hypothetical protein	-
SRAA_RS01875	389920..390198	-	279	WP_045530666.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	381766..390406	8640
	-	flank	IS/Tn	-	-	387831..389063	1232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (37-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-86)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12374.43 Da
• Isoelectric Point: 6.9497

>T30663 WP_197538516.1 NZ_AP014568:c385391-385059 [Serpentinomonas raichei]
MEVMVFVELPIFIRCAAELFADEDIAALQNTLLENPAAGDLIPGGRGLRKLRVPLPGRGKRSGARVIYYHWVSKEQCYLI
YAYAKNVSTNLTPVQLRRLAEIMQAEIPDE

Nucleotide

• Length: 333 bp

>T30663 NZ_AP014568:c385391-385059 [Serpentinomonas raichei]
ATGGAAGTCATGGTTTTTGTGGAACTTCCGATCTTCATCCGCTGTGCAGCCGAACTGTTCGCGGATGAGGATATCGCCGC
GCTGCAGAACACCTTGCTGGAAAATCCCGCCGCTGGCGACTTGATTCCCGGCGGCAGGGGGCTGCGCAAGTTGCGTGTTC
CGTTGCCTGGCAGGGGCAAGCGCAGTGGGGCACGGGTCATCTACTACCACTGGGTGAGCAAGGAGCAGTGCTACTTGATC
TATGCCTATGCGAAAAATGTCAGCACCAATCTGACACCCGTTCAACTGCGGCGGTTGGCCGAGATCATGCAAGCGGAGAT
ACCAGATGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10831.45 Da
• Isoelectric Point: 10.5808

>AT30663 WP_045530658.1 NZ_AP014568:c385066-384776 [Serpentinomonas raichei]
MNDQQFDELLDSVRDMGRHMRGEKLLGVRVQAFPEPDVKAIRERTGLSQSRFAHLIGVKPKTLQNWEQHRVRPAGPARAL
LKIVEANPDALSALHA

Nucleotide

• Length: 291 bp

>AT30663 NZ_AP014568:c385066-384776 [Serpentinomonas raichei]
ATGAATGACCAACAGTTCGACGAGTTGCTCGACAGCGTGCGCGACATGGGGCGCCATATGCGCGGCGAGAAGCTGCTGGG
CGTTCGCGTGCAGGCGTTTCCTGAGCCCGATGTCAAGGCGATCCGTGAGCGCACCGGTCTGTCGCAGAGTCGTTTCGCCC
ACCTGATCGGCGTCAAGCCCAAGACCCTGCAGAACTGGGAGCAACATCGCGTGCGTCCAGCAGGACCTGCGCGCGCCTTG
CTAAAGATCGTCGAAGCCAACCCTGATGCGCTCTCGGCGCTTCACGCATAG