Detailed information of TA system TA030652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2628714..2629318
• Replicon: chromosome
• Accession: NZ_AP014545
• Organism: Amphritea japonica ATCC BAA-1530

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7R6PEV1
• Locus tag: AMJAP_RS12225
• Protein ID: WP_019620268.1
• Coordinates: 2629001..2629318 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AMJAP_RS12220
• Protein ID: WP_019620269.1
• Coordinates: 2628714..2629004 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMJAP_RS12200	2624403..2625206	-	804	WP_019620274.1	sel1 repeat family protein	-
AMJAP_RS12205	2625399..2625899	-	501	WP_019620273.1	hypothetical protein	-
AMJAP_RS12210	2627000..2627476	-	477	WP_019620271.1	YHS domain-containing protein	-
AMJAP_RS12215	2627612..2628493	+	882	WP_019620270.1	AraC family transcriptional regulator	-
AMJAP_RS12220	2628714..2629004	-	291	WP_019620269.1	helix-turn-helix domain-containing protein	Antitoxin
AMJAP_RS12225	2629001..2629318	-	318	WP_019620268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMJAP_RS12230	2629779..2630693	-	915	WP_156815099.1	response regulator	-
AMJAP_RS12235	2630727..2634188	-	3462	WP_019620266.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (3-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12091.07 Da
• Isoelectric Point: 9.8875

>T30652 WP_019620268.1 NZ_AP014545:c2629318-2629001 [Amphritea japonica ATCC BAA-1530]
MVIVETAVFTKLIKEMLSDDEYKNLQEVLISRPASGDLIKESGGLRKIRWSVQGTGKSGGIRVIYYWVVDDHHIRMLYVY
PKGKQDNLTKAQLAQLKAIVERWQQ

Nucleotide

• Length: 318 bp

>T30652 NZ_AP014545:c2629318-2629001 [Amphritea japonica ATCC BAA-1530]
ATGGTTATTGTCGAAACCGCTGTATTCACAAAGCTCATCAAAGAGATGCTGAGTGATGACGAATATAAAAACCTCCAGGA
AGTGCTTATAAGTCGTCCAGCTTCAGGGGATCTGATAAAAGAATCCGGCGGGCTTCGGAAAATCCGCTGGAGTGTACAAG
GGACGGGGAAAAGTGGTGGTATACGCGTAATCTACTATTGGGTAGTTGATGACCATCATATTCGCATGTTGTATGTATAC
CCTAAGGGCAAACAAGACAACTTAACTAAAGCACAATTGGCACAATTGAAAGCCATAGTTGAAAGGTGGCAACAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10982.47 Da
• Isoelectric Point: 5.7643

>AT30652 WP_019620269.1 NZ_AP014545:c2629004-2628714 [Amphritea japonica ATCC BAA-1530]
MNNEMFDELLESVQQMDEIVKENKKASRTFDFPDPEVKAIRERLGVSQEKFAFILGVSKRTIENWEQGRRHPTGAARSLL
RVVEADPEHALKALSA

Nucleotide

• Length: 291 bp

>AT30652 NZ_AP014545:c2629004-2628714 [Amphritea japonica ATCC BAA-1530]
ATGAATAATGAAATGTTTGATGAGCTTTTAGAAAGCGTTCAGCAGATGGATGAGATTGTAAAAGAAAATAAAAAAGCATC
TCGGACCTTTGATTTTCCCGACCCTGAAGTTAAGGCTATTCGTGAGCGTTTAGGAGTTTCTCAGGAAAAGTTTGCATTCA
TTCTCGGCGTGAGTAAACGCACAATAGAAAACTGGGAGCAAGGGCGTCGACACCCCACGGGAGCAGCGCGATCACTTTTG
CGGGTTGTCGAAGCGGATCCAGAACATGCGCTTAAAGCACTGAGTGCATAA

Structures

Toxin

• AlphaFold DB: A0A7R6PEV1