Detailed information of TA system TA030641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 377143..377758
• Replicon: chromosome
• Accession: NZ_AP014525
• Organism: Vibrio cholerae MS6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: MS6_RS15420
• Protein ID: WP_001162670.1
• Coordinates: 377143..377430 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS6_RS15425
• Protein ID: WP_001232701.1
• Coordinates: 377441..377758 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6_RS15390	372635..372892	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MS6_RS15395	372880..373182	+	303	WP_000229318.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MS6_RS19515	373471..373695	+	225	WP_001894423.1	DUF3709 domain-containing protein	-
MS6_RS19520	374139..374258	+	120	WP_071919600.1	DUF645 family protein	-
MS6_RS20145	374262..374315	+	54	Protein_369	DUF645 family protein	-
MS6_RS15405	374917..375921	+	1005	WP_000964931.1	LD-carboxypeptidase	-
MS6_RS15415	376696..376876	+	181	Protein_371	DUF645 family protein	-
MS6_RS15420	377143..377430	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS6_RS15425	377441..377758	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
MS6_RS20395	377755..377865	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
MS6_RS19545	377983..378261	+	279	WP_001921601.1	DUF3709 domain-containing protein	-
MS6_RS20160	378513..378719	+	207	Protein_376	DUF3709 domain-containing protein	-
MS6_RS19555	378919..379020	+	102	WP_001921603.1	hypothetical protein	-
MS6_RS15430	379010..379396	+	387	WP_000703163.1	VOC family protein	-
MS6_RS15435	379591..379842	+	252	WP_001890111.1	TIGR03643 family protein	-
MS6_RS19560	379815..379964	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
MS6_RS15440	380078..380521	+	444	WP_000803902.1	hypothetical protein	-
MS6_RS19565	380525..380635	+	111	WP_071908336.1	DUF3265 domain-containing protein	-
MS6_RS15445	380665..381528	+	864	WP_000030911.1	hypothetical protein	-
MS6_RS15450	381771..382049	+	279	WP_000280319.1	DUF3709 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	297729..445581	147852
	-	inside	Integron	qnrVC4	-	302809..445205	142396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T30641 WP_001162670.1 NZ_AP014525:377143-377430 [Vibrio cholerae MS6]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T30641 NZ_AP014525:377143-377430 [Vibrio cholerae MS6]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT30641 WP_001232701.1 NZ_AP014525:377441-377758 [Vibrio cholerae MS6]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT30641 NZ_AP014525:377441-377758 [Vibrio cholerae MS6]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36