Detailed information of TA system TA030631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4440019..4440673
• Replicon: chromosome
• Accession: NZ_AP014522
• Organism: Pseudomonas protegens Cab57

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PPC_RS19950
• Protein ID: WP_041119058.1
• Coordinates: 4440019..4440369 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PPC_RS19955
• Protein ID: WP_041119060.1
• Coordinates: 4440356..4440673 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPC_RS19940	4436778..4438310	+	1533	WP_019092840.1	NADH-quinone oxidoreductase subunit M	-
PPC_RS19945	4438318..4439781	+	1464	WP_015636299.1	NADH-quinone oxidoreductase subunit NuoN	-
PPC_RS19950	4440019..4440369	+	351	WP_041119058.1	hypothetical protein	Toxin
PPC_RS19955	4440356..4440673	+	318	WP_041119060.1	transcriptional regulator	Antitoxin
PPC_RS19960	4440742..4442124	-	1383	WP_041119061.1	cysteine--tRNA ligase	-
PPC_RS19965	4442143..4443843	-	1701	WP_041119063.1	glutamine--tRNA ligase/YqeY domain fusion protein	-
PPC_RS19970	4444114..4444617	+	504	WP_011062201.1	peptidyl-prolyl cis-trans isomerase	-
PPC_RS19975	4444614..4445366	+	753	WP_011062202.1	UDP-2,3-diacylglucosamine diphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-101)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13955.12 Da
• Isoelectric Point: 9.9922

>T30631 WP_041119058.1 NZ_AP014522:4440019-4440369 [Pseudomonas protegens Cab57]
MNALFIELPAFARYRKGFLDDELFCELQIELLRRPHAGVLIQGTGGLRKMRFIDERRNKGRRGGLRVIYYWWSGGAQFWL
FTLFDKEQMDDLLPQQRQQLKQLLEREIEARRHDQT

Nucleotide

• Length: 351 bp

>T30631 NZ_AP014522:4440019-4440369 [Pseudomonas protegens Cab57]
ATGAATGCTCTGTTTATTGAACTACCGGCATTTGCGCGATACCGAAAGGGCTTCCTTGACGACGAATTGTTCTGCGAACT
GCAGATCGAGTTGCTGCGCCGCCCGCACGCGGGTGTTCTGATTCAGGGCACTGGCGGCTTGCGCAAGATGCGTTTCATCG
ATGAGCGACGCAACAAGGGCAGGCGTGGCGGGCTGAGGGTGATTTATTACTGGTGGTCCGGAGGAGCACAGTTCTGGCTC
TTCACCCTGTTCGACAAGGAGCAGATGGACGACCTGCTACCGCAGCAGCGACAACAGCTCAAACAACTGCTGGAACGTGA
AATCGAGGCCAGAAGACATGACCAAACGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12084.94 Da
• Isoelectric Point: 10.4439

>AT30631 WP_041119060.1 NZ_AP014522:4440356-4440673 [Pseudomonas protegens Cab57]
MTKRDIFSELVEGIDALTQERQGKITLRSHKVKFDKLPPITAAEVMNIRQQLNLSRSVFAMYLRTNTRTLENWEQGRAIP
NAQATTLIRLVERYPDTVERLASLA

Nucleotide

• Length: 318 bp

>AT30631 NZ_AP014522:4440356-4440673 [Pseudomonas protegens Cab57]
ATGACCAAACGTGACATCTTTTCCGAACTGGTTGAAGGCATCGACGCCCTCACCCAGGAGCGCCAGGGCAAGATCACCCT
GCGCAGCCATAAAGTGAAATTCGACAAGCTGCCCCCCATCACCGCCGCCGAGGTGATGAACATCCGCCAGCAACTCAACC
TCTCGCGCTCGGTATTCGCCATGTACCTGCGCACCAACACCCGCACCCTGGAGAACTGGGAACAGGGCCGCGCCATACCC
AACGCCCAGGCCACGACCTTGATACGCCTGGTGGAGCGCTACCCCGATACAGTGGAGCGCCTCGCCTCGCTGGCCTGA