Detailed information of TA system TA030623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1908892..1909538
• Replicon: chromosome
• Accession: NZ_AP014520
• Organism: Aggregatibacter actinomycetemcomitans NUM4039 strain NUM 4039

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: AANUM_RS09525
• Protein ID: WP_005555126.1
• Coordinates: 1909188..1909538 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: AANUM_RS09520
• Protein ID: WP_005540827.1
• Coordinates: 1908892..1909191 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AANUM_RS09515 (AANUM_1927)	1905923..1908856	+	2934	WP_005555125.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
AANUM_RS09520 (AANUM_1928)	1908892..1909191	-	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
AANUM_RS09525 (AANUM_1929)	1909188..1909538	-	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AANUM_RS09530 (AANUM_1930)	1909725..1911308	-	1584	WP_005540820.1	peptide chain release factor 3	-
AANUM_RS09535 (AANUM_1931)	1911665..1912492	+	828	WP_005540818.1	hypothetical protein	-
AANUM_RS09540 (AANUM_1932)	1912580..1913917	+	1338	WP_005540817.1	argininosuccinate synthase	-
AANUM_RS09545 (AANUM_1933)	1914108..1914353	+	246	WP_005540815.1	YgjV family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T30623 WP_005555126.1 NZ_AP014520:c1909538-1909188 [Aggregatibacter actinomycetemcomitans NUM4039]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T30623 NZ_AP014520:c1909538-1909188 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGTGGGCGGTGATTTTAACGGAACGATTTGAAACTTGGTTAAATCAACAGCCGATAAAAACGCAGGAATGTGTTTTGGC
AAACTTGGTACGGTTGGAACATTTTGGTCCGAATTTAGCCCGTCCTTATGTAGATAGCGTAGCAGGGTCAATTCATAGCA
ACATGAAAGAACTACGCGTTCAGCATTCGGGAAGAGCAATTCGACTCTTTTTTGCTTTTGATCCAAAGCGTAGAGCCATT
GTTTTATGTGGTGGAGATAAAGGCAATGACAAGCGGTTTTACCGTACGATGATTAGAATTGCAGATCAAGAATTGAATCA
TTATTTGGCAACCATGGAGCAAACAAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT30623 WP_005540827.1 NZ_AP014520:c1909191-1908892 [Aggregatibacter actinomycetemcomitans NUM4039]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT30623 NZ_AP014520:c1909191-1908892 [Aggregatibacter actinomycetemcomitans NUM4039]
ATGAAAACATTACAACAAGTTATTGATGAACGTTCGGTAGAAAGCCGCCAACGTATTCAAACCATGGCGGAAGATTTAAT
TCTTGAAACCGGTTTGCAACTGTTGCGAGAAGAATTAAATATATCGCAACAACAATTAGCTACGATTTTAGGTGTAACTC
AACCTGCAATTAATCAACTGGAACAGCGTGGCAATGAAATTAAGCTTGCAACCCTCAAACGTTACATCGAAGCAATGGGC
GGTAAATTACGCCTTAGTGTAGAAATGCCTGATGGACATCAGCGGGTTTTTCAAATTTAA

Structures

Toxin

• AlphaFold DB: X2JQ23

Antitoxin

• AlphaFold DB: X2JT85