Detailed information of TA system TA030599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5116444..5117097
• Replicon: chromosome
• Accession: NZ_AP013063
• Organism: Serratia marcescens SM39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: SM39_RS23745
• Protein ID: WP_033645671.1
• Coordinates: 5116444..5116794 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: SM39_RS23750
• Protein ID: WP_019453270.1
• Coordinates: 5116798..5117097 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM39_RS23710	5111620..5112030	-	411	WP_041037740.1	GNAT family N-acetyltransferase	-
SM39_RS23715	5112059..5113429	-	1371	WP_041037742.1	glutamine synthetase family protein	-
SM39_RS23720	5113675..5113992	+	318	WP_041037744.1	hypothetical protein	-
SM39_RS23725	5113983..5114378	-	396	WP_041037746.1	hypothetical protein	-
SM39_RS23730	5114392..5114784	-	393	WP_041037748.1	hypothetical protein	-
SM39_RS23735	5114812..5115201	-	390	WP_033652822.1	hypothetical protein	-
SM39_RS23740	5115201..5116172	-	972	Protein_4747	hypothetical protein	-
SM39_RS23745	5116444..5116794	+	351	WP_033645671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM39_RS23750	5116798..5117097	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
SM39_RS23755	5117087..5118004	-	918	WP_033648568.1	LysR family transcriptional regulator	-
SM39_RS23760	5118173..5118871	+	699	WP_033648567.1	CoA transferase subunit A	-
SM39_RS23765	5118883..5119536	+	654	WP_041037752.1	CoA transferase subunit B	-
SM39_RS23770	5119550..5120734	+	1185	WP_041037754.1	acetyl-CoA C-acetyltransferase	-
SM39_RS23775	5120753..5121676	+	924	WP_041037756.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.81 Da
• Isoelectric Point: 8.2699

>T30599 WP_033645671.1 NZ_AP013063:5116444-5116794 [Serratia marcescens SM39]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T30599 NZ_AP013063:5116444-5116794 [Serratia marcescens SM39]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAGCAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCCGGGCCTTCTCTCCGGCGGCCTTTTGTCGACGTGCTGAAAGGTTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATTGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT30599 WP_019453270.1 NZ_AP013063:5116798-5117097 [Serratia marcescens SM39]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT30599 NZ_AP013063:5116798-5117097 [Serratia marcescens SM39]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCGGTCGCGGCCATCGAAGGGCGCGGCAACGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3