Detailed information of TA system TA030457

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60797..61036
• Replicon: plasmid pRSB225
• Accession: NC_025100
• Organism: uncultured bacterium

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: pRSB225_81
• Protein ID: YP_009066038.1
• Coordinates: 60845..61036 (+)
• Length: 64 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	60797..60857 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
pRSB225_77	58270..59073	+	804	YP_009066034.1	conjugal transfer pilus acetylation protein TraX	-
pRSB225_78	59128..59688	+	561	YP_009066035.1	conjugal transfer fertility inhibition protein FinO	-
pRSB225_79	59819..60031	+	213	YP_009066036.1	hypothetical protein	-
pRSB225_80	59904..60086	+	183	YP_009066037.1	hypothetical protein	-
-	60797..60857	-	61	-	-	Antitoxin
pRSB225_81	60845..61036	+	192	YP_009066038.1	leader region of the killer mRNA	Toxin
pRSB225_82	60902..61036	+	135	YP_009066039.1	SrnB'-	-
pRSB225_83	61333..61587	+	255	YP_009066040.1	replication regulatory protein repA2	-
pRSB225_84	61824..61898	+	75	YP_009066041.1	putative replication protein	-
pRSB225_85	61891..62748	+	858	YP_009066042.1	replication initiation protein A	-
pRSB225_86	62954..63379	+	426	YP_009066043.1	transposase	-
pRSB225_87	63376..63726	+	351	YP_009066044.1	transposase	-
pRSB225_88	63757..65370	+	1614	YP_009066045.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / mph(A) / dfrA14 / catA1 / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..164550	164550
	-	inside	IScluster/Tn	-	-	62954..68695	5741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-63)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7138.59 Da
• Isoelectric Point: 8.1393

>T30457 YP_009066038.1 NC_025100:60845-61036 [uncultured bacterium]
MMHFSTTDCSLFFAKMSKFMTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 192 bp

>T30457 NC_025100:60845-61036 [uncultured bacterium]
ATGATGCACTTTAGCACTACTGATTGTAGCCTCTTCTTTGCAAAGATGTCAAAGTTTATGACGAAATATACCCTTATCGG
GTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT30457 NC_025100:c60857-60797 [uncultured bacterium]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin