Detailed information of TA system TA030442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5656539..5657134
• Replicon: chromosome
• Accession: NC_023149
• Organism: Pseudomonas aeruginosa SCV20265

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: SCV20265_RS32425
• Protein ID: WP_003113526.1
• Coordinates: 5656856..5657134 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SCV20265_RS26410
• Protein ID: WP_023122809.1
• Coordinates: 5656539..5656844 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCV20265_RS32405	5651877..5653313	+	1437	WP_023122806.1	HAD-IA family hydrolase	-
SCV20265_RS32410	5653317..5654276	+	960	WP_078459576.1	DNA-protecting protein DprA	-
SCV20265_RS32415	5654311..5655183	-	873	WP_024100099.1	DUF4868 domain-containing protein	-
SCV20265_RS26410	5656539..5656844	-	306	WP_023122809.1	HigA family addiction module antidote protein	Antitoxin
SCV20265_RS32425	5656856..5657134	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SCV20265_RS26415	5657463..5659691	+	2229	WP_003099265.1	TonB-dependent receptor	-
SCV20265_RS26420	5659761..5660408	-	648	WP_003095021.1	carbonate dehydratase	-
SCV20265_RS26425	5660470..5661708	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T30442 WP_003113526.1 NC_023149:c5657134-5656856 [Pseudomonas aeruginosa SCV20265]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T30442 NC_023149:c5657134-5656856 [Pseudomonas aeruginosa SCV20265]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11279.87 Da
• Isoelectric Point: 5.0747

>AT30442 WP_023122809.1 NC_023149:c5656844-5656539 [Pseudomonas aeruginosa SCV20265]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTMNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT30442 NC_023149:c5656844-5656539 [Pseudomonas aeruginosa SCV20265]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAATGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3