Detailed information of TA system TA030434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 264386..265050
• Replicon: plasmid unnamed
• Accession: NC_023144
• Organism: Rhodococcus pyridinivorans SB3094

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: Y013_RS25075
• Protein ID: WP_041804319.1
• Coordinates: 264697..265050 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: V9XQ84
• Locus tag: Y013_RS25070
• Protein ID: WP_024100396.1
• Coordinates: 264386..264691 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y013_RS27070	259472..259663	-	192	WP_024100391.1	hypothetical protein	-
Y013_RS26585	260205..261001	+	797	WP_162164140.1	IS5 family transposase	-
Y013_RS25055	261819..263144	+	1326	WP_144377038.1	hypothetical protein	-
Y013_RS25770	263564..263830	+	267	WP_051424989.1	hypothetical protein	-
Y013_RS25065	263894..264190	+	297	WP_144377039.1	hypothetical protein	-
Y013_RS25070	264386..264691	-	306	WP_024100396.1	XRE family transcriptional regulator	Antitoxin
Y013_RS25075	264697..265050	-	354	WP_041804319.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y013_RS25080	265350..265601	-	252	WP_144377040.1	hypothetical protein	-
Y013_RS25085	265630..265896	-	267	WP_158455947.1	AAA family ATPase	-
Y013_RS26590	265850..266144	+	295	Protein_250	4Fe-4S dicluster domain-containing protein	-
Y013_RS25090	266158..267198	-	1041	WP_024100401.1	NAD(P)-dependent alcohol dehydrogenase	-
Y013_RS25095	268451..268900	-	450	WP_024100402.1	hypothetical protein	-
Y013_RS25100	268979..269203	+	225	WP_041804424.1	cold-shock protein	-
Y013_RS25105	269210..269977	+	768	WP_024100404.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..361397	361397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-87)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13624.58 Da
• Isoelectric Point: 5.2494

>T30434 WP_041804319.1 NC_023144:c265050-264697 [Rhodococcus pyridinivorans SB3094]
MKWVVDVERIEAWLTQLDDNSYDQVIAALELLAENGPMLGRPLVDTVETSRHSNMKELRPGSRGRSELRILFAFDPKRSA
IMLIAGDKSGQWKKWYKVNIPIADDLFDEHLEQWERK

Nucleotide

• Length: 354 bp

>T30434 NC_023144:c265050-264697 [Rhodococcus pyridinivorans SB3094]
ATGAAATGGGTGGTGGACGTCGAGCGAATCGAGGCGTGGTTGACACAACTGGACGACAATTCGTACGACCAGGTGATCGC
TGCACTCGAGCTGCTTGCGGAGAACGGCCCCATGTTGGGGCGGCCCTTGGTGGACACCGTGGAAACCTCACGTCACTCGA
ACATGAAAGAACTACGACCGGGATCACGTGGACGATCCGAACTGCGAATTCTTTTCGCCTTCGACCCGAAGCGGTCGGCG
ATCATGTTGATCGCCGGGGACAAATCAGGACAGTGGAAGAAGTGGTACAAGGTGAACATTCCAATAGCCGATGACCTCTT
CGACGAACATCTCGAGCAGTGGGAAAGGAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11618.33 Da
• Isoelectric Point: 10.5706

>AT30434 WP_024100396.1 NC_023144:c264691-264386 [Rhodococcus pyridinivorans SB3094]
MAKSLDDMLAQRPANREKVDAHKQRMRSQVRAYKLKELRKELLLSQGQVADRLGVGQNRISNIERGDLDRVRLDTLKRYV
DAVGGSLRIEVEVGDNRYQIV

Nucleotide

• Length: 306 bp

>AT30434 NC_023144:c264691-264386 [Rhodococcus pyridinivorans SB3094]
ATGGCTAAGAGTCTTGACGATATGTTGGCTCAGCGACCGGCCAACCGTGAGAAGGTAGACGCTCACAAGCAGCGGATGCG
CTCGCAGGTTCGCGCGTACAAACTGAAGGAACTTCGGAAAGAGCTTCTGCTCTCCCAGGGCCAGGTCGCCGACCGTCTCG
GTGTCGGACAGAACCGGATCTCGAACATCGAGCGCGGCGATCTCGATCGGGTGCGGCTCGACACTCTGAAGCGGTACGTC
GACGCCGTGGGTGGATCGCTTCGGATCGAAGTCGAGGTGGGCGACAACCGGTACCAAATCGTCTGA

Structures

Antitoxin

• AlphaFold DB: V9XQ84