Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 5816299..5816954 Replicon chromosome
Accession NC_023064
Organism Pseudomonas sp. TKP

Toxin (Protein)


Gene name higB Uniprot ID A0A855K4I9
Locus tag U771_RS25915 Protein ID WP_024077334.1
Coordinates 5816610..5816954 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A855K566
Locus tag U771_RS25910 Protein ID WP_024077333.1
Coordinates 5816299..5816613 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U771_RS25890 5812525..5813538 + 1014 WP_024077329.1 GGDEF domain-containing protein -
U771_RS25895 5813756..5815006 + 1251 WP_024077330.1 aspartate aminotransferase family protein -
U771_RS25905 5815523..5816239 + 717 WP_024077332.1 hypothetical protein -
U771_RS25910 5816299..5816613 - 315 WP_024077333.1 helix-turn-helix domain-containing protein Antitoxin
U771_RS25915 5816610..5816954 - 345 WP_024077334.1 toxin HigB-2 Toxin
U771_RS25920 5817048..5818139 - 1092 WP_024077335.1 pyridoxal phosphate-dependent aminotransferase -
U771_RS25925 5818287..5819174 - 888 WP_024077336.1 heme o synthase -
U771_RS25930 5819186..5819521 - 336 WP_003176003.1 cytochrome o ubiquinol oxidase subunit IV -
U771_RS25935 5819521..5820147 - 627 WP_024077337.1 cytochrome o ubiquinol oxidase subunit III -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(42-94)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13327.19 Da        Isoelectric Point: 9.8860

>T30410 WP_024077334.1 NC_023064:c5816954-5816610 [Pseudomonas sp. TKP]
MRSIFFETSSFTATVGHYLTDDEYRLLQNEMQWNPLAGDVMPRTGGFRKLRWSDGKRGKGKRSGLRVIYYWLTTDGHFWM
FAIYNKDELENLTADQEKALKKAIEAELKKRGTP

Download         Length: 345 bp

>T30410 NC_023064:c5816954-5816610 [Pseudomonas sp. TKP]
ATGCGTTCTATTTTCTTCGAGACTTCATCGTTTACCGCCACCGTAGGTCATTACCTGACGGATGATGAATATCGATTGCT
GCAAAACGAGATGCAGTGGAACCCTTTGGCTGGCGATGTGATGCCCCGTACAGGAGGGTTTCGCAAGCTACGGTGGTCAG
ATGGCAAGCGTGGTAAAGGAAAGCGTAGTGGCTTACGGGTTATTTACTACTGGCTCACCACTGATGGTCACTTTTGGATG
TTTGCTATCTACAACAAAGACGAATTGGAAAATTTGACTGCCGATCAAGAGAAGGCACTTAAAAAGGCCATAGAGGCCGA
ACTGAAAAAACGAGGTACCCCATGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11789.63 Da        Isoelectric Point: 10.3067

>AT30410 WP_024077333.1 NC_023064:c5816613-5816299 [Pseudomonas sp. TKP]
MKKRDLFAELMQGVSDMTAHREGKITLRQSTVEDKPVPEVSAQEIVALRDKLHMSQSVFARSIRTSPGTLRNWEQDKSKP
NAQAALLIRLVEKFPDMVDRLATV

Download         Length: 315 bp

>AT30410 NC_023064:c5816613-5816299 [Pseudomonas sp. TKP]
ATGAAAAAGCGCGATCTGTTTGCAGAGTTGATGCAGGGCGTTAGCGACATGACTGCTCACCGAGAAGGCAAAATCACTCT
TCGGCAAAGCACTGTGGAGGACAAGCCCGTTCCTGAGGTGTCAGCCCAAGAGATCGTGGCACTGCGGGATAAATTGCATA
TGTCACAGTCGGTGTTTGCCCGGAGCATACGGACGAGCCCCGGCACGCTGAGAAACTGGGAACAGGACAAATCCAAACCC
AATGCTCAGGCCGCACTCCTGATCAGGCTGGTTGAAAAGTTTCCGGATATGGTGGATCGATTGGCGACCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855K4I9

Antitoxin

Source ID Structure
AlphaFold DB A0A855K566

References