Detailed information of TA system TA030380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 109968..110578
• Replicon: chromosome
• Accession: NC_023032
• Organism: Cronobacter sakazakii CMCC 45402

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V5TV61
• Locus tag: P262_RS00475
• Protein ID: WP_007779945.1
• Coordinates: 109968..110288 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: P262_RS00480
• Protein ID: WP_007779948.1
• Coordinates: 110288..110578 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P262_RS00460	106964..108115	+	1152	WP_023897762.1	L-threonine dehydrogenase	-
P262_RS00465	108140..109012	-	873	WP_023897763.1	ROK family protein	-
P262_RS00470	109169..109768	+	600	WP_007779941.1	LysE family transporter	-
P262_RS00475	109968..110288	+	321	WP_007779945.1	hypothetical protein	Toxin
P262_RS00480	110288..110578	+	291	WP_007779948.1	helix-turn-helix domain-containing protein	Antitoxin
P262_RS00485	110652..111581	-	930	WP_023897767.1	formate dehydrogenase accessory protein FdhE	-
P262_RS00490	111578..112213	-	636	WP_004385165.1	formate dehydrogenase cytochrome b556 subunit	-
P262_RS00495	112210..113112	-	903	WP_004385164.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12396.41 Da
• Isoelectric Point: 10.2290

>T30380 WP_007779945.1 NC_023032:109968-110288 [Cronobacter malonaticus]
MLFIETEIFTRAVAALLSDDDYQRLQRLQWFLALRPDYGDVIPDTGGLRKARWLAGGRGKRGGVRIIYFYRAAADEIRLL
LIYRKGIKDDLSPHEKSVLRMLNERW

Nucleotide

• Length: 321 bp

>T30380 NC_023032:109968-110288 [Cronobacter malonaticus]
ATGCTCTTCATTGAAACGGAAATCTTTACCCGGGCCGTGGCCGCCCTGCTCAGTGATGACGATTACCAGCGGTTGCAGCG
GTTGCAGTGGTTCCTGGCCCTGCGGCCCGATTACGGTGATGTTATCCCGGATACCGGCGGGCTTCGTAAAGCTCGCTGGC
TTGCAGGCGGACGGGGCAAACGAGGCGGCGTCAGGATCATCTATTTTTATCGCGCTGCTGCCGATGAAATCCGCCTGCTG
CTGATTTATCGCAAGGGGATAAAAGACGATCTCTCGCCTCACGAAAAATCTGTGCTCAGAATGCTGAATGAGAGGTGGTG
A

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10674.16 Da
• Isoelectric Point: 10.4419

>AT30380 WP_007779948.1 NC_023032:110288-110578 [Cronobacter malonaticus]
MDNALFEQLTESLRQMNDISEGRREPSRAFVIDALKIKEIRQASGLSQTKFASLISVSVDTLRNWEQGRRSPTGPARALL
RAIANDPQHVISALAL

Nucleotide

• Length: 291 bp

>AT30380 NC_023032:110288-110578 [Cronobacter malonaticus]
ATGGACAACGCGCTGTTTGAACAGTTAACCGAAAGCCTGCGGCAGATGAATGATATCAGCGAAGGCCGCCGCGAGCCTTC
ACGGGCTTTTGTCATCGACGCGCTGAAAATAAAAGAGATCAGACAGGCATCAGGGCTTTCTCAGACGAAATTTGCCAGCC
TGATTTCGGTAAGTGTGGATACGCTGCGCAACTGGGAGCAGGGAAGGCGTTCGCCGACGGGGCCCGCGCGTGCGCTGTTG
CGTGCGATTGCGAACGATCCGCAGCATGTTATCTCTGCACTGGCGTTGTAA

Structures

Toxin

• AlphaFold DB: V5TV61