Detailed information of TA system TA030376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5508101..5508696
• Replicon: chromosome
• Accession: NC_023019
• Organism: Pseudomonas aeruginosa MTB-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: U769_RS31530
• Protein ID: WP_079279175.1
• Coordinates: 5508418..5508696 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S3KUN4
• Locus tag: U769_RS25495
• Protein ID: WP_003111575.1
• Coordinates: 5508101..5508406 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U769_RS25470	5503135..5503569	-	435	WP_003140508.1	hypothetical protein	-
U769_RS25480	5504085..5504375	-	291	Protein_5080	DUF5447 family protein	-
U769_RS25485	5504379..5504573	-	195	WP_034044419.1	hypothetical protein	-
U769_RS25490	5504587..5504859	-	273	WP_003115921.1	hypothetical protein	-
U769_RS31525	5505313..5506929	+	1617	WP_023876008.1	DUF262 domain-containing protein	-
U769_RS31920	5506856..5507746	+	891	WP_144096443.1	DUF4935 domain-containing protein	-
U769_RS25495	5508101..5508406	-	306	WP_003111575.1	HigA family addiction module antidote protein	Antitoxin
U769_RS31530	5508418..5508696	-	279	WP_079279175.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
U769_RS25500	5509025..5511253	+	2229	WP_023876009.1	TonB-dependent receptor	-
U769_RS25505	5511323..5511970	-	648	WP_003095021.1	carbonate dehydratase	-
U769_RS25510	5512032..5513270	-	1239	WP_023107901.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10676.22 Da
• Isoelectric Point: 7.2451

>T30376 WP_079279175.1 NC_023019:c5508696-5508418 [Pseudomonas aeruginosa MTB-1]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNWLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T30376 NC_023019:c5508696-5508418 [Pseudomonas aeruginosa MTB-1]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACTGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11205.72 Da
• Isoelectric Point: 4.4475

>AT30376 WP_003111575.1 NC_023019:c5508406-5508101 [Pseudomonas aeruginosa MTB-1]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT30376 NC_023019:c5508406-5508101 [Pseudomonas aeruginosa MTB-1]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Structures

Antitoxin

• AlphaFold DB: A0A0S3KUN4