Detailed information of TA system TA030375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4727093..4727773
• Replicon: chromosome
• Accession: NC_023019
• Organism: Pseudomonas aeruginosa MTB-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9E8L2H3
• Locus tag: U769_RS21910
• Protein ID: WP_023875759.1
• Coordinates: 4727420..4727773 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: U769_RS21905
• Protein ID: WP_003111824.1
• Coordinates: 4727093..4727416 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U769_RS31340	4722443..4722672	+	230	Protein_4365	helix-turn-helix transcriptional regulator	-
U769_RS31880	4722749..4723822	-	1074	WP_023875757.1	hypothetical protein	-
U769_RS21890	4724886..4725467	+	582	WP_023105512.1	hypothetical protein	-
U769_RS31345	4725461..4726030	-	570	Protein_4368	tyrosine-type recombinase/integrase	-
U769_RS31350	4726241..4726480	-	240	WP_144096440.1	integrase	-
U769_RS21900	4726480..4726812	-	333	WP_041025703.1	hypothetical protein	-
U769_RS31885	4726867..4726974	+	108	Protein_4371	IS5/IS1182 family transposase	-
U769_RS21905	4727093..4727416	-	324	WP_003111824.1	XRE family transcriptional regulator	Antitoxin
U769_RS21910	4727420..4727773	-	354	WP_023875759.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
U769_RS21915	4728037..4728276	-	240	WP_044060930.1	hypothetical protein	-
U769_RS21920	4728483..4728755	+	273	WP_009315277.1	hypothetical protein	-
U769_RS21925	4728786..4729211	-	426	WP_003111827.1	ring-cleaving dioxygenase	-
U769_RS21930	4729312..4730196	+	885	WP_023875760.1	LysR family transcriptional regulator	-
U769_RS21935	4730169..4731122	-	954	WP_003085661.1	LysR family transcriptional regulator	-
U769_RS21940	4731343..4731777	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4674062..4730196	56134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13581.81 Da
• Isoelectric Point: 4.7017

>T30375 WP_023875759.1 NC_023019:c4727773-4727420 [Pseudomonas aeruginosa MTB-1]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSDINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLYDEHLHQLREED

Nucleotide

• Length: 354 bp

>T30375 NC_023019:c4727773-4727420 [Pseudomonas aeruginosa MTB-1]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGACATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGACTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11887.72 Da
• Isoelectric Point: 5.8900

>AT30375 WP_003111824.1 NC_023019:c4727416-4727093 [Pseudomonas aeruginosa MTB-1]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT30375 NC_023019:c4727416-4727093 [Pseudomonas aeruginosa MTB-1]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGTCAGCTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA