30366

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2623220..2623816 Replicon chromosome
Accession NC_022997
Organism Hyphomicrobium nitrativorans NL23

Toxin (Protein)


Gene name graT Uniprot ID V5SEY0
Locus tag W911_RS12190 Protein ID WP_023787852.1
Coordinates 2623220..2623501 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V5SEV0
Locus tag W911_RS12195 Protein ID WP_023787853.1
Coordinates 2623517..2623816 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
W911_RS12165 2618579..2620813 + 2235 WP_023787847.1 PBP1A family penicillin-binding protein -
W911_RS12170 2620820..2621488 + 669 WP_081717722.1 DUF1214 domain-containing protein -
W911_RS12175 2621491..2622111 + 621 WP_023787849.1 hypothetical protein -
W911_RS12185 2622560..2622751 - 192 WP_023787851.1 hypothetical protein -
W911_RS12190 2623220..2623501 + 282 WP_023787852.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
W911_RS12195 2623517..2623816 + 300 WP_023787853.1 HigA family addiction module antidote protein Antitoxin
W911_RS12200 2623911..2624258 - 348 WP_023787854.1 DUF1491 family protein -
W911_RS12205 2624315..2625010 - 696 WP_023787855.1 peptidoglycan-binding protein -
W911_RS12210 2625302..2625652 + 351 WP_023787856.1 hypothetical protein -
W911_RS12215 2625795..2626223 + 429 WP_023787857.1 SufE family protein -
W911_RS12220 2626239..2626793 - 555 WP_023787858.1 hypothetical protein -
W911_RS17870 2626860..2627357 - 498 WP_023787859.1 chromosomal replication initiator protein DnaA -
W911_RS18485 2627563..2627895 + 333 WP_023787860.1 hypothetical protein -
W911_RS12235 2627937..2628344 - 408 WP_023787861.1 MucR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(14-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10521.14 Da        Isoelectric Point: 10.3737

>T30366 WP_023787852.1 NC_022997:2623220-2623501 [Hyphomicrobium nitrativorans NL23]
MIKSYRNAMTEAVARGKAPKGFPADLARRALLRLLALDHAKTLDDLRSPPGNRLEALKGDRKGQHSIRINAQWRICFVWT
EAGPEQVEIVDYH

Download         Length: 282 bp

>T30366 NC_022997:2623220-2623501 [Hyphomicrobium nitrativorans NL23]
ATGATCAAGAGCTACCGGAACGCGATGACTGAGGCGGTCGCCAGGGGAAAAGCGCCGAAGGGCTTCCCGGCCGACCTCGC
AAGGCGCGCACTTCTAAGGCTCTTGGCCCTGGATCACGCGAAGACCCTTGATGACCTTCGGAGCCCACCGGGAAACCGCC
TCGAAGCTCTCAAAGGCGACCGCAAGGGGCAGCACTCGATCCGCATCAACGCCCAGTGGCGCATCTGTTTCGTGTGGACC
GAAGCCGGACCGGAACAGGTTGAAATTGTCGATTACCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11128.00 Da        Isoelectric Point: 9.6236

>AT30366 WP_023787853.1 NC_022997:2623517-2623816 [Hyphomicrobium nitrativorans NL23]
MNKSILPPIHPGEYLREEFLVPLKLSPGALAKRLGVPRTRIERIAAEQTGITTDTALRLAKFFKTSPEFWISMQTGYDLK
IAEKALKTQLDQIEEMSAA

Download         Length: 300 bp

>AT30366 NC_022997:2623517-2623816 [Hyphomicrobium nitrativorans NL23]
ATGAACAAGAGCATCTTGCCTCCAATCCATCCCGGAGAATACTTGCGCGAGGAATTCCTTGTTCCGCTCAAATTGAGCCC
CGGCGCCCTCGCAAAACGCCTCGGCGTACCCCGCACACGGATCGAGCGCATCGCTGCCGAACAGACTGGGATCACGACCG
ACACCGCGTTGCGTCTGGCGAAGTTCTTCAAGACCTCGCCTGAGTTCTGGATAAGTATGCAGACGGGCTACGATCTCAAG
ATCGCCGAGAAGGCGCTGAAAACGCAACTCGACCAGATCGAGGAGATGAGCGCGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V5SEY0

Antitoxin

Source ID Structure
AlphaFold DB V5SEV0

References