Detailed information of TA system TA030366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2623220..2623816
• Replicon: chromosome
• Accession: NC_022997
• Organism: Hyphomicrobium nitrativorans NL23

Toxin (Protein)

• Gene name: graT
• Uniprot ID: V5SEY0
• Locus tag: W911_RS12190
• Protein ID: WP_023787852.1
• Coordinates: 2623220..2623501 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V5SEV0
• Locus tag: W911_RS12195
• Protein ID: WP_023787853.1
• Coordinates: 2623517..2623816 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
W911_RS12165	2618579..2620813	+	2235	WP_023787847.1	PBP1A family penicillin-binding protein	-
W911_RS12170	2620820..2621488	+	669	WP_081717722.1	DUF1214 domain-containing protein	-
W911_RS12175	2621491..2622111	+	621	WP_023787849.1	hypothetical protein	-
W911_RS12185	2622560..2622751	-	192	WP_023787851.1	hypothetical protein	-
W911_RS12190	2623220..2623501	+	282	WP_023787852.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
W911_RS12195	2623517..2623816	+	300	WP_023787853.1	HigA family addiction module antidote protein	Antitoxin
W911_RS12200	2623911..2624258	-	348	WP_023787854.1	DUF1491 family protein	-
W911_RS12205	2624315..2625010	-	696	WP_023787855.1	peptidoglycan-binding protein	-
W911_RS12210	2625302..2625652	+	351	WP_023787856.1	hypothetical protein	-
W911_RS12215	2625795..2626223	+	429	WP_023787857.1	SufE family protein	-
W911_RS12220	2626239..2626793	-	555	WP_023787858.1	hypothetical protein	-
W911_RS17870	2626860..2627357	-	498	WP_023787859.1	chromosomal replication initiator protein DnaA	-
W911_RS18485	2627563..2627895	+	333	WP_023787860.1	hypothetical protein	-
W911_RS12235	2627937..2628344	-	408	WP_023787861.1	MucR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (14-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10521.14 Da
• Isoelectric Point: 10.3737

>T30366 WP_023787852.1 NC_022997:2623220-2623501 [Hyphomicrobium nitrativorans NL23]
MIKSYRNAMTEAVARGKAPKGFPADLARRALLRLLALDHAKTLDDLRSPPGNRLEALKGDRKGQHSIRINAQWRICFVWT
EAGPEQVEIVDYH

Nucleotide

• Length: 282 bp

>T30366 NC_022997:2623220-2623501 [Hyphomicrobium nitrativorans NL23]
ATGATCAAGAGCTACCGGAACGCGATGACTGAGGCGGTCGCCAGGGGAAAAGCGCCGAAGGGCTTCCCGGCCGACCTCGC
AAGGCGCGCACTTCTAAGGCTCTTGGCCCTGGATCACGCGAAGACCCTTGATGACCTTCGGAGCCCACCGGGAAACCGCC
TCGAAGCTCTCAAAGGCGACCGCAAGGGGCAGCACTCGATCCGCATCAACGCCCAGTGGCGCATCTGTTTCGTGTGGACC
GAAGCCGGACCGGAACAGGTTGAAATTGTCGATTACCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11128.00 Da
• Isoelectric Point: 9.6236

>AT30366 WP_023787853.1 NC_022997:2623517-2623816 [Hyphomicrobium nitrativorans NL23]
MNKSILPPIHPGEYLREEFLVPLKLSPGALAKRLGVPRTRIERIAAEQTGITTDTALRLAKFFKTSPEFWISMQTGYDLK
IAEKALKTQLDQIEEMSAA

Nucleotide

• Length: 300 bp

>AT30366 NC_022997:2623517-2623816 [Hyphomicrobium nitrativorans NL23]
ATGAACAAGAGCATCTTGCCTCCAATCCATCCCGGAGAATACTTGCGCGAGGAATTCCTTGTTCCGCTCAAATTGAGCCC
CGGCGCCCTCGCAAAACGCCTCGGCGTACCCCGCACACGGATCGAGCGCATCGCTGCCGAACAGACTGGGATCACGACCG
ACACCGCGTTGCGTCTGGCGAAGTTCTTCAAGACCTCGCCTGAGTTCTGGATAAGTATGCAGACGGGCTACGATCTCAAG
ATCGCCGAGAAGGCGCTGAAAACGCAACTCGACCAGATCGAGGAGATGAGCGCGGCATAA

Structures

Toxin

• AlphaFold DB: V5SEY0

Antitoxin

• AlphaFold DB: V5SEV0