Detailed information of TA system TA030326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5454224..5454819
• Replicon: chromosome
• Accession: NC_022808
• Organism: Pseudomonas aeruginosa PA1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PA1S_RS31010
• Protein ID: WP_003113526.1
• Coordinates: 5454541..5454819 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA1S_RS25325
• Protein ID: WP_003099268.1
• Coordinates: 5454224..5454529 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA1S_RS25290 (PA1S_25300)	5449364..5450212	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
PA1S_RS25300 (PA1S_25310)	5450379..5451320	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
PA1S_RS25305 (PA1S_25315)	5451437..5452051	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
PA1S_RS25310 (PA1S_25320)	5452093..5452677	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
PA1S_RS25315 (PA1S_25325)	5452718..5453818	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
PA1S_RS25325 (PA1S_25335)	5454224..5454529	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
PA1S_RS31010	5454541..5454819	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA1S_RS25330 (PA1S_25340)	5455148..5457376	+	2229	WP_003099265.1	TonB-dependent receptor	-
PA1S_RS25335 (PA1S_25345)	5457446..5458093	-	648	WP_003095021.1	carbonate dehydratase	-
PA1S_RS25340 (PA1S_25350)	5458155..5459393	-	1239	WP_009316205.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T30326 WP_003113526.1 NC_022808:c5454819-5454541 [Pseudomonas aeruginosa PA1]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T30326 NC_022808:c5454819-5454541 [Pseudomonas aeruginosa PA1]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT30326 WP_003099268.1 NC_022808:c5454529-5454224 [Pseudomonas aeruginosa PA1]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT30326 NC_022808:c5454529-5454224 [Pseudomonas aeruginosa PA1]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3