Detailed information of TA system TA030266

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24523..24792
• Replicon: plasmid pJJ1886_5
• Accession: NC_022651
• Organism: Escherichia coli JJ1886

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: P423_RS26465
• Protein ID: WP_001312861.1
• Coordinates: 24634..24792 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24523..24588 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P423_RS26435	20316..20843	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
P423_RS26440	20899..21132	+	234	WP_000006004.1	DUF905 domain-containing protein	-
P423_RS26445	21191..23149	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
P423_RS26450	23204..23638	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
P423_RS26455	23635..24354	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
P423_RS30375	24366..24554	-	189	WP_001299721.1	hypothetical protein	-
-	24366..24590	+	225	NuclAT_0	-	-
-	24366..24590	+	225	NuclAT_0	-	-
-	24366..24590	+	225	NuclAT_0	-	-
-	24366..24590	+	225	NuclAT_0	-	-
-	24523..24588	+	66	-	-	Antitoxin
P423_RS26465	24634..24792	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
P423_RS26470	25143..25847	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
P423_RS26475	25900..27795	+	1896	Protein_31	Tn3 family transposase	-
P423_RS26480	27894..28547	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
P423_RS30290	28640..28897	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
P423_RS26490	28830..29231	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B	-	1..110040	110040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T30266 WP_001312861.1 NC_022651:24634-24792 [Escherichia coli JJ1886]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T30266 NC_022651:24634-24792 [Escherichia coli JJ1886]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT30266 NC_022651:24523-24588 [Escherichia coli JJ1886]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin