Detailed information of TA system TA030257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4432022..4432624
• Replicon: chromosome
• Accession: NC_022648
• Organism: Escherichia coli JJ1886

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: P423_RS22370
• Protein ID: WP_000897302.1
• Coordinates: 4432022..4432333 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: P423_RS22375
• Protein ID: WP_000356397.1
• Coordinates: 4432334..4432624 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P423_RS22345	4427936..4428535	+	600	WP_001296610.1	glucose-1-phosphatase	-
P423_RS22350	4428529..4429401	+	873	WP_000920762.1	virulence factor BrkB family protein	-
P423_RS22355	4429398..4429835	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
P423_RS22360	4429880..4430821	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
P423_RS22365	4430885..4431793	-	909	WP_001385591.1	alpha/beta hydrolase	-
P423_RS22370	4432022..4432333	+	312	WP_000897302.1	hypothetical protein	Toxin
P423_RS22375	4432334..4432624	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
P423_RS22385	4432983..4433261	+	279	WP_001296612.1	hypothetical protein	-
P423_RS22390	4433658..4433876	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
P423_RS22395	4434061..4434510	-	450	WP_032140890.1	hypothetical protein	-
P423_RS22400	4434826..4435674	-	849	WP_001038650.1	hypothetical protein	-
P423_RS22405	4435964..4436206	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
P423_RS22410	4436388..4437317	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T30257 WP_000897302.1 NC_022648:4432022-4432333 [Escherichia coli JJ1886]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T30257 NC_022648:4432022-4432333 [Escherichia coli JJ1886]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT30257 WP_000356397.1 NC_022648:4432334-4432624 [Escherichia coli JJ1886]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT30257 NC_022648:4432334-4432624 [Escherichia coli JJ1886]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416