Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3581088..3581815 Replicon chromosome
Accession NC_022648
Organism Escherichia coli JJ1886

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag P423_RS18115 Protein ID WP_000550189.1
Coordinates 3581501..3581815 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P423_RS18110 Protein ID WP_000560269.1
Coordinates 3581088..3581504 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P423_RS18100 3576448..3578799 + 2352 WP_000695432.1 alpha-glucosidase -
P423_RS18105 3579025..3581043 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
P423_RS18110 3581088..3581504 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
P423_RS18115 3581501..3581815 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
P423_RS18125 3582100..3583236 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
P423_RS18130 3583321..3583824 + 504 WP_001295542.1 M48 family metallopeptidase -
P423_RS18135 3583901..3584593 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
P423_RS18140 3584672..3585658 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T30254 WP_000550189.1 NC_022648:c3581815-3581501 [Escherichia coli JJ1886]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T30254 NC_022648:c3581815-3581501 [Escherichia coli JJ1886]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT30254 WP_000560269.1 NC_022648:c3581504-3581088 [Escherichia coli JJ1886]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT30254 NC_022648:c3581504-3581088 [Escherichia coli JJ1886]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References