Detailed information of TA system TA030250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2290010..2290512
• Replicon: chromosome
• Accession: NC_022648
• Organism: Escherichia coli JJ1886

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: P423_RS11930
• Protein ID: WP_000767819.1
• Coordinates: 2290010..2290264 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: P423_RS11935
• Protein ID: WP_001259253.1
• Coordinates: 2290261..2290512 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P423_RS11905	2285828..2286286	-	459	WP_023363206.1	IS200/IS605 family transposase	-
P423_RS11915	2286503..2287861	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
P423_RS29990	2287851..2287913	-	63	WP_010723108.1	membrane protein YoeI	-
P423_RS11920	2288128..2289057	-	930	WP_000803366.1	LysR family transcriptional regulator	-
P423_RS11925	2289103..2289927	-	825	WP_000754737.1	SDR family oxidoreductase	-
P423_RS11930	2290010..2290264	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
P423_RS11935	2290261..2290512	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
P423_RS27870	2290795..2290845	+	51	WP_001364200.1	his operon leader peptide	-
P423_RS11940	2290991..2291890	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
P423_RS11945	2291896..2293200	+	1305	WP_001296211.1	histidinol dehydrogenase	-
P423_RS11950	2293197..2294267	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
P423_RS11955	2294267..2295334	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2285828..2286286	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T30250 WP_000767819.1 NC_022648:c2290264-2290010 [Escherichia coli JJ1886]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T30250 NC_022648:c2290264-2290010 [Escherichia coli JJ1886]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT30250 WP_001259253.1 NC_022648:c2290512-2290261 [Escherichia coli JJ1886]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT30250 NC_022648:c2290512-2290261 [Escherichia coli JJ1886]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3