Detailed information of TA system TA030193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 520176..520782
• Replicon: chromosome
• Accession: NC_022576
• Organism: Candidatus Symbiobacter mobilis CR

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U5N5P7
• Locus tag: CENROD_RS02100
• Protein ID: WP_022771416.1
• Coordinates: 520176..520511 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CENROD_RS02105
• Protein ID: WP_041193195.1
• Coordinates: 520495..520782 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CENROD_RS02075	516133..516618	-	486	WP_022771410.1	colanic acid biosynthesis glycosyl transferase	-
CENROD_RS02080	517012..518517	-	1506	WP_022771411.1	ISNCY family transposase	-
CENROD_RS12935	518752..519000	-	249	WP_081699909.1	DUF2442 domain-containing protein	-
CENROD_RS14035	519013..519165	-	153	WP_202961171.1	DUF4160 domain-containing protein	-
CENROD_RS02090	519437..519655	-	219	Protein_420	UDP-N-acetylglucosamine 2-epimerase	-
CENROD_RS02095	519606..520019	-	414	Protein_421	capsular polysaccharide biosynthesis protein CapF	-
CENROD_RS02100	520176..520511	+	336	WP_022771416.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CENROD_RS02105	520495..520782	+	288	WP_041193195.1	helix-turn-helix transcriptional regulator	Antitoxin
CENROD_RS02110	521099..522397	-	1299	WP_041193196.1	type II toxin-antitoxin system HipA family toxin	-
CENROD_RS02115	522387..522725	-	339	WP_022771419.1	helix-turn-helix domain-containing protein	-
CENROD_RS13805	522744..523193	-	450	WP_022771420.1	colanic acid biosynthesis glycosyl transferase	-
CENROD_RS02125	523223..524359	-	1137	WP_022771421.1	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	-
CENROD_RS02130	524538..525350	+	813	WP_022771422.1	Rpn family recombination-promoting nuclease/putative transposase	-
CENROD_RS02135	525389..525637	+	249	WP_022771423.1	BrnA antitoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-90)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12596.70 Da
• Isoelectric Point: 9.7518

>T30193 WP_022771416.1 NC_022576:520176-520511 [Candidatus Symbiobacter mobilis CR]
MEYAIDYYSEGVEQEILALPDTLAARYVVLTRRMVAVGPNLGEPHTKSMGDGLLELRLKGAEGIARVFYCTQVGRRIVML
HSFIKKTSRTPPRELEIALNRMKEVKHGHTR

Nucleotide

• Length: 336 bp

>T30193 NC_022576:520176-520511 [Candidatus Symbiobacter mobilis CR]
ATGGAATACGCGATTGATTACTACAGCGAAGGCGTGGAGCAAGAAATCCTGGCGCTGCCAGACACCTTGGCGGCGCGCTA
TGTTGTCTTGACGCGGCGCATGGTGGCTGTTGGTCCGAACCTTGGAGAGCCGCACACCAAGTCGATGGGGGATGGCCTGC
TGGAACTGCGCCTCAAGGGCGCGGAAGGCATTGCCCGGGTCTTCTACTGCACGCAAGTGGGCCGGCGCATCGTGATGCTG
CACAGCTTCATCAAGAAGACCAGCCGCACACCACCGCGTGAACTAGAAATTGCACTGAACCGCATGAAAGAGGTGAAACA
TGGCCACACACGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10310.95 Da
• Isoelectric Point: 9.1046

>AT30193 WP_041193195.1 NC_022576:520495-520782 [Candidatus Symbiobacter mobilis CR]
MATHDEVIKKLMSRPGVAAEVERIEREEGELLDALLKARHEAGLTQAELAARMGTQAPAVARLERALATGKHSPSVATLR
KYVKACGKELHLQVS

Nucleotide

• Length: 288 bp

>AT30193 NC_022576:520495-520782 [Candidatus Symbiobacter mobilis CR]
ATGGCCACACACGATGAAGTCATCAAGAAGCTGATGAGCCGTCCCGGCGTAGCCGCCGAAGTTGAACGCATTGAGCGCGA
AGAAGGGGAGCTTCTGGATGCGCTGCTCAAAGCGCGCCACGAGGCGGGCTTGACCCAGGCCGAGCTTGCCGCCCGCATGG
GCACGCAAGCCCCTGCCGTTGCCCGTCTTGAGCGTGCACTGGCCACGGGCAAGCACTCGCCCTCGGTCGCCACCCTCAGA
AAGTACGTCAAGGCCTGCGGTAAGGAGTTGCACCTACAGGTGTCATGA

Structures

Toxin

• AlphaFold DB: U5N5P7