Detailed information of TA system TA030173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1829841..1830477
• Replicon: chromosome
• Accession: NC_022545
• Organism: Rhizobium sp. IRBG74

Toxin (Protein)

• Gene name: graT
• Uniprot ID: U4QGN3
• Locus tag: BN877_RS21985
• Protein ID: WP_022563816.1
• Coordinates: 1829841..1830122 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BN877_RS21990
• Protein ID: WP_020009806.1
• Coordinates: 1830178..1830477 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN877_RS21965	1825874..1826881	+	1008	WP_022563812.1	iron-siderophore ABC transporter substrate-binding protein	-
BN877_RS21970	1826878..1827921	+	1044	WP_022563813.1	iron ABC transporter permease	-
BN877_RS21975	1827924..1828958	+	1035	WP_022563814.1	iron chelate uptake ABC transporter family permease subunit	-
BN877_RS21980	1828955..1829779	+	825	WP_022563815.1	ABC transporter ATP-binding protein	-
BN877_RS21985	1829841..1830122	+	282	WP_022563816.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BN877_RS21990	1830178..1830477	+	300	WP_020009806.1	HigA family addiction module antidote protein	Antitoxin
BN877_RS21995	1830497..1831369	-	873	WP_022563817.1	helix-turn-helix transcriptional regulator	-
BN877_RS22000	1831503..1832717	+	1215	WP_022563818.1	amino acid ABC transporter substrate-binding protein	-
BN877_RS22005	1832788..1833660	+	873	WP_045533916.1	branched-chain amino acid ABC transporter permease	-
BN877_RS22010	1833657..1834565	+	909	WP_022563820.1	branched-chain amino acid ABC transporter permease	-
BN877_RS22015	1834562..1835290	+	729	WP_022563821.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10548.99 Da
• Isoelectric Point: 9.0298

>T30173 WP_022563816.1 NC_022545:1829841-1830122 [Rhizobium pusense]
MIRSFKNKLTASIDDGSVKKGFPADLVRRAQQLLTILDAATTVEDLRSPPGNRLEKLSGDREGQYSIRLNKQWRICFVWT
QAGPESVEITDYH

Nucleotide

• Length: 282 bp

>T30173 NC_022545:1829841-1830122 [Rhizobium pusense]
ATGATCCGTTCTTTCAAAAACAAGCTGACCGCCTCAATTGACGACGGATCTGTGAAGAAGGGCTTTCCCGCCGATCTGGT
ACGCCGCGCCCAGCAGCTGCTCACGATCCTGGATGCGGCGACCACGGTAGAAGACCTTCGCTCACCGCCCGGCAATCGTT
TGGAAAAGCTGTCCGGCGATCGTGAGGGCCAATATTCGATCCGGCTCAACAAGCAGTGGCGCATCTGCTTCGTCTGGACG
CAAGCAGGGCCGGAAAGCGTGGAAATAACAGACTATCACTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11072.92 Da
• Isoelectric Point: 8.5148

>AT30173 WP_020009806.1 NC_022545:1830178-1830477 [Rhizobium pusense]
MTISKSPPIHPGEILRELYLEPLGMSAYALARHLNIPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
AAAVRLEADLANIKELEAA

Nucleotide

• Length: 300 bp

>AT30173 NC_022545:1830178-1830477 [Rhizobium pusense]
ATGACAATCAGTAAATCGCCACCGATCCATCCGGGCGAAATCCTGCGGGAACTCTATCTTGAGCCGCTCGGGATGTCGGC
TTACGCTCTTGCAAGGCACCTCAATATTCCGCGCACCCGCATAGAACGGATTGCGGGAGAGAAACTTGGTATCACCGCCG
ACACCGCCTTGCGCCTTGCAAAGTTTTTCAAGACCACACCGGAATATTGGCTGAACTTGCAGAACGCCTTTGACATTAAG
GCGGCGGCTGTGAGGCTGGAAGCGGACCTTGCCAATATCAAGGAACTGGAAGCGGCCTAA

Structures

Toxin

• AlphaFold DB: U4QGN3