Detailed information of TA system TA030107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4223241..4223843
• Replicon: chromosome
• Accession: NC_022364
• Organism: Escherichia coli LY180

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LY180_RS20885
• Protein ID: WP_000897305.1
• Coordinates: 4223241..4223552 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LY180_RS20890
• Protein ID: WP_000356397.1
• Coordinates: 4223553..4223843 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY180_RS20855	4218271..4219056	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
LY180_RS20860	4219155..4219754	+	600	WP_001315111.1	glucose-1-phosphatase	-
LY180_RS20865	4219748..4220620	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LY180_RS20870	4220617..4221054	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
LY180_RS20875	4221099..4222040	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LY180_RS20880	4222104..4223012	-	909	WP_001162704.1	alpha/beta hydrolase	-
LY180_RS20885	4223241..4223552	+	312	WP_000897305.1	hypothetical protein	Toxin
LY180_RS20890	4223553..4223843	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
LY180_RS20895	4224448..4224666	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
LY180_RS20900	4224885..4225127	+	243	WP_001086388.1	hypothetical protein	-
LY180_RS20910	4225457..4226386	-	930	WP_000027702.1	formate dehydrogenase accessory protein FdhE	-
LY180_RS20915	4226383..4227018	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LY180_RS20920	4227015..4227917	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T30107 WP_000897305.1 NC_022364:4223241-4223552 [Escherichia coli LY180]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T30107 NC_022364:4223241-4223552 [Escherichia coli LY180]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT30107 WP_000356397.1 NC_022364:4223553-4223843 [Escherichia coli LY180]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT30107 NC_022364:4223553-4223843 [Escherichia coli LY180]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7