Detailed information of TA system TA030085

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1451254..1451625
• Replicon: chromosome
• Accession: NC_022364
• Organism: Escherichia coli LY180

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: LY180_RS07115
• Protein ID: WP_001317028.1
• Coordinates: 1451431..1451625 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1451254..1451432 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY180_RS07090	1447209..1448582	+	1374	WP_000123740.1	ATP-dependent RNA helicase DbpA	-
LY180_RS07095	1448711..1449646	-	936	WP_001157422.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LY180_RS24505	1449698..1450933	-	1236	WP_000040852.1	site-specific integrase	-
LY180_RS07105	1450935..1451150	-	216	WP_000079604.1	excisionase XisR	-
LY180_RS07110	1451229..1451438	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1451254..1451432	+	179	NuclAT_0	-	Antitoxin
-	1451254..1451432	+	179	NuclAT_0	-	Antitoxin
-	1451254..1451432	+	179	NuclAT_0	-	Antitoxin
-	1451254..1451432	+	179	NuclAT_0	-	Antitoxin
LY180_RS07115	1451431..1451625	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
LY180_RS07120	1451682..1452491	-	810	WP_000166319.1	recombination protein RecT	-
LY180_RS07125	1452484..1455084	-	2601	WP_000105152.1	exodeoxyribonuclease VIII	-
LY180_RS07130	1455186..1455461	-	276	WP_001349884.1	hypothetical protein	-
LY180_RS07135	1455536..1455706	-	171	WP_001349883.1	hypothetical protein	-
LY180_RS07140	1455706..1455927	-	222	WP_000560220.1	killing protein KilR	-
LY180_RS23955	1456348..1456500	-	153	WP_001169153.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1449698..1498554	48856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T30085 WP_001317028.1 NC_022364:c1451625-1451431 [Escherichia coli LY180]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T30085 NC_022364:c1451625-1451431 [Escherichia coli LY180]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGGTATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT30085 NC_022364:1451254-1451432 [Escherichia coli LY180]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin