Detailed information of TA system TA030077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 307013..307622
• Replicon: chromosome
• Accession: NC_022357
• Organism: Sulfuricella denitrificans skB26

Toxin (Protein)

• Gene name: graT
• Uniprot ID: S6AHY1
• Locus tag: SCD_RS01440
• Protein ID: WP_009206916.1
• Coordinates: 307344..307622 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: SCD_RS01435
• Protein ID: WP_009206917.1
• Coordinates: 307013..307318 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCD_RS01410	302699..303523	+	825	WP_009206921.1	39S ribosomal protein L45	-
SCD_RS01415	303584..304198	+	615	WP_009206920.1	hypothetical protein	-
SCD_RS01420	304198..305742	+	1545	WP_009206919.1	ubiquinone biosynthesis regulatory protein kinase UbiB	-
SCD_RS01430	305778..307007	-	1230	WP_198408616.1	TIGR03862 family flavoprotein	-
SCD_RS01435	307013..307318	-	306	WP_009206917.1	HigA family addiction module antidote protein	Antitoxin
SCD_RS01440	307344..307622	-	279	WP_009206916.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SCD_RS01445	307719..308051	-	333	WP_009206915.1	hypothetical protein	-
SCD_RS01450	308120..308374	-	255	WP_009206914.1	DUF2442 domain-containing protein	-
SCD_RS01455	308367..308585	-	219	WP_009206913.1	DUF4160 domain-containing protein	-
SCD_RS01460	308760..309176	-	417	WP_009206912.1	VOC family protein	-
SCD_RS01465	309173..310027	-	855	WP_009206911.1	hypothetical protein	-
SCD_RS01470	310118..310417	-	300	WP_009206910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10736.09 Da
• Isoelectric Point: 7.0497

>T30077 WP_009206916.1 NC_022357:c307622-307344 [Sulfuricella denitrificans skB26]
MIHSFACSDTEALFHSQDVPRFKNIERVARRKLLQLHAATELNSLQVPPGNRLEALKDDRKSQHSIRVNDQWRVCFVWQE
DGAHSVEIVDYH

Nucleotide

• Length: 279 bp

>T30077 NC_022357:c307622-307344 [Sulfuricella denitrificans skB26]
ATGATTCATTCATTCGCCTGCTCCGATACCGAGGCGCTATTCCATAGCCAGGACGTACCCCGGTTCAAGAACATCGAACG
GGTTGCACGTCGCAAGCTGCTTCAACTGCATGCTGCCACCGAGCTGAACAGCTTGCAAGTGCCTCCAGGGAACCGCTTGG
AAGCGTTGAAAGACGACCGCAAGAGTCAACACAGCATTCGTGTCAACGATCAATGGCGGGTGTGCTTCGTGTGGCAGGAG
GATGGCGCCCATAGTGTGGAAATTGTTGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11458.35 Da
• Isoelectric Point: 7.0139

>AT30077 WP_009206917.1 NC_022357:c307318-307013 [Sulfuricella denitrificans skB26]
MTKLLEEIHPGEILLEDFMKPMGITARQLAADIDVSPSRISDIVNGSRPVTADTALRLGIFFGMEPRFWLNLQSEYDMRI
ATRNLTDKIAPRIRVFQRVAA

Nucleotide

• Length: 306 bp

>AT30077 NC_022357:c307318-307013 [Sulfuricella denitrificans skB26]
ATGACAAAACTGCTTGAAGAGATTCACCCCGGCGAAATTCTCCTTGAGGATTTCATGAAGCCTATGGGAATTACAGCCAG
ACAACTGGCTGCTGACATTGATGTTTCGCCTAGCCGTATTAGTGATATTGTCAACGGATCACGGCCTGTTACTGCCGACA
CTGCGTTGCGTCTCGGAATATTCTTCGGAATGGAGCCACGTTTCTGGCTAAACCTGCAAAGCGAGTACGACATGCGGATA
GCAACGCGCAATCTCACAGACAAAATTGCGCCTCGTATTCGAGTTTTTCAACGGGTTGCAGCATAA

Structures

Toxin

• AlphaFold DB: S6AHY1