Detailed information of TA system TA029985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1302614..1303173
• Replicon: chromosome
• Accession: NC_022234
• Organism: Variovorax paradoxus B4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: T1XNX9
• Locus tag: VAPA_RS33265
• Protein ID: WP_021004654.1
• Coordinates: 1302904..1303173 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VAPA_RS33260
• Protein ID: WP_021004653.1
• Coordinates: 1302614..1302862 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VAPA_RS34320	1297908..1298435	+	528	WP_021004650.1	DUF1232 domain-containing protein	-
VAPA_RS33245	1298335..1299906	-	1572	WP_196232596.1	phosphatase PAP2 family protein	-
VAPA_RS34990	1299813..1300337	-	525	WP_051255459.1	hypothetical protein	-
VAPA_RS33250	1300457..1301608	+	1152	Protein_1254	IS5 family transposase	-
VAPA_RS33260	1302614..1302862	-	249	WP_021004653.1	HigA family addiction module antidote protein	Antitoxin
VAPA_RS33265	1302904..1303173	-	270	WP_021004654.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VAPA_RS33270	1304007..1304951	+	945	WP_041946745.1	DUF1738 domain-containing protein	-
VAPA_RS33275	1305343..1305723	+	381	WP_021004656.1	LysR family transcriptional regulator	-
VAPA_RS33280	1306007..1306327	-	321	WP_051255477.1	hypothetical protein	-
VAPA_RS34330	1306736..1307290	+	555	WP_196232597.1	ATP-binding protein	-
VAPA_RS33285	1307180..1307818	-	639	WP_021004659.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1212674..1347340	134666

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10268.79 Da
• Isoelectric Point: 8.0356

>T29985 WP_021004654.1 NC_022234:c1303173-1302904 [Variovorax paradoxus B4]
MAIQSFSCPDTEELFTTGKNARFANIRDVAIRKLTQLDAAPTVVFMKMPPGNDLKFYDGAWHVRIKQQWRLIFKWGDAGP
YDVRIDDPH

Nucleotide

• Length: 270 bp

>T29985 NC_022234:c1303173-1302904 [Variovorax paradoxus B4]
ATGGCGATTCAATCCTTCTCTTGCCCGGATACTGAAGAGCTTTTCACCACGGGCAAAAACGCTCGCTTCGCCAATATCCG
GGACGTGGCGATCCGCAAGCTGACCCAGTTGGACGCTGCGCCGACGGTAGTTTTCATGAAGATGCCACCAGGCAACGACC
TGAAGTTTTACGACGGCGCCTGGCACGTCAGGATCAAGCAGCAGTGGCGGCTGATCTTCAAATGGGGCGATGCGGGCCCA
TACGACGTGAGGATTGATGACCCGCACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9160.52 Da
• Isoelectric Point: 7.2008

>AT29985 WP_021004653.1 NC_022234:c1302862-1302614 [Variovorax paradoxus B4]
MFKNGMRPVHPGEVLREDYLKPLGMTANALAKALHVPASRVNDIVLERRGISPDTALRLTRYFGGEEADAVGWINMQATY
DF

Nucleotide

• Length: 249 bp

>AT29985 NC_022234:c1302862-1302614 [Variovorax paradoxus B4]
ATGTTTAAGAATGGAATGCGCCCGGTCCATCCCGGCGAAGTCCTCCGCGAGGACTACCTCAAGCCCCTTGGCATGACTGC
CAACGCGCTGGCGAAGGCGCTGCACGTGCCTGCGTCCCGTGTCAATGACATCGTGCTCGAACGCCGCGGCATTTCGCCAG
ACACTGCGCTGCGCCTGACTCGCTACTTTGGCGGTGAAGAGGCCGACGCGGTGGGTTGGATCAATATGCAAGCGACCTAC
GATTTCTAG

Structures

Toxin

• AlphaFold DB: T1XNX9