Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2384188..2384705 Replicon chromosome
Accession NC_022222
Organism Staphylococcus aureus subsp. aureus 6850

Toxin (Protein)


Gene name yoeB Uniprot ID A0A2S6D1N9
Locus tag RSAU_RS12020 Protein ID WP_000113263.1
Coordinates 2384188..2384454 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID T1YB60
Locus tag RSAU_RS12025 Protein ID WP_000584499.1
Coordinates 2384454..2384705 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RSAU_RS11995 2379392..2379928 - 537 WP_020977539.1 GNAT family N-acetyltransferase -
RSAU_RS12000 2380398..2381222 - 825 WP_000594640.1 formate/nitrite transporter family protein -
RSAU_RS12005 2381425..2381607 - 183 WP_000079140.1 hypothetical protein -
RSAU_RS12010 2381694..2382161 - 468 WP_020977540.1 SRPBCC domain-containing protein -
RSAU_RS12015 2382347..2383894 - 1548 WP_020977541.1 zinc ABC transporter substrate-binding lipoprotein AdcA -
RSAU_RS12020 2384188..2384454 - 267 WP_000113263.1 Txe/YoeB family addiction module toxin Toxin
RSAU_RS12025 2384454..2384705 - 252 WP_000584499.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RSAU_RS12030 2384878..2384970 - 93 Protein_2272 hypothetical protein -
RSAU_RS12035 2384977..2385576 - 600 WP_020977542.1 DsbA family protein -
RSAU_RS12040 2385595..2385957 - 363 WP_000819843.1 DUF4467 domain-containing protein -
RSAU_RS12045 2386212..2387462 + 1251 WP_020977543.1 FemA/FemB family glycyltransferase FmhA -
RSAU_RS12050 2387559..2388290 - 732 WP_000615461.1 amino acid ABC transporter ATP-binding protein -
RSAU_RS12055 2388287..2389006 - 720 WP_000479576.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(8-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10437.01 Da        Isoelectric Point: 10.1628

>T29961 WP_000113263.1 NC_022222:c2384454-2384188 [Staphylococcus aureus subsp. aureus 6850]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALKGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T29961 NC_022222:c2384454-2384188 [Staphylococcus aureus subsp. aureus 6850]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGCGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9433.60 Da        Isoelectric Point: 4.3407

>AT29961 WP_000584499.1 NC_022222:c2384705-2384454 [Staphylococcus aureus subsp. aureus 6850]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT29961 NC_022222:c2384705-2384454 [Staphylococcus aureus subsp. aureus 6850]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACGCTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S6D1N9

Antitoxin

Source ID Structure
PDB 6L8G
PDB 6L8F
PDB 7BWF
PDB 6L8E
AlphaFold DB T1YB60

References