Detailed information of TA system TA029897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 73453..73988
• Replicon: plasmid p1
• Accession: NC_021987
• Organism: Enterococcus faecium Aus0085

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q3Y221
• Locus tag: EFAU085_RS15100
• Protein ID: WP_002305108.1
• Coordinates: 73453..73725 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A133CED7
• Locus tag: EFAU085_RS15105
• Protein ID: WP_002303154.1
• Coordinates: 73725..73988 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFAU085_RS15070 (EFAU085_p1083)	68460..69647	-	1188	WP_002303156.1	NADP-dependent malic enzyme	-
EFAU085_RS15075 (EFAU085_p1084)	69834..70781	+	948	WP_002303155.1	sugar-binding domain-containing protein	-
EFAU085_RS15080 (EFAU085_p1085)	71078..71626	-	549	WP_002292681.1	hypothetical protein	-
EFAU085_RS15085 (EFAU085_p1086)	71627..72484	-	858	WP_002292680.1	ParA family protein	-
EFAU085_RS15090 (EFAU085_p1087)	72770..73057	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EFAU085_RS15095 (EFAU085_p1088)	73047..73376	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
EFAU085_RS15100 (EFAU085_p1089)	73453..73725	-	273	WP_002305108.1	Txe/YoeB family addiction module toxin	Toxin
EFAU085_RS15105 (EFAU085_p1090)	73725..73988	-	264	WP_002303154.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EFAU085_RS15110 (EFAU085_p1091)	74094..74417	-	324	WP_224453712.1	hypothetical protein	-
EFAU085_RS15115 (EFAU085_p1092)	74516..75079	-	564	Protein_79	IS6-like element ISS1N family transposase	-
EFAU085_RS15125 (EFAU085_p1094)	75159..76321	+	1163	WP_134771592.1	IS3 family transposase	-
EFAU085_RS15130 (EFAU085_p1095)	76792..77691	-	900	WP_002303235.1	aldose 1-epimerase family protein	-
EFAU085_RS15135 (EFAU085_p1096)	77712..77984	-	273	WP_002305107.1	DUF3884 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..130716	130716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10806.28 Da
• Isoelectric Point: 9.9080

>T29897 WP_002305108.1 NC_021987:c73725-73453 [Enterococcus faecium Aus0085]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T29897 NC_021987:c73725-73453 [Enterococcus faecium Aus0085]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9886.16 Da
• Isoelectric Point: 4.5370

>AT29897 WP_002303154.1 NC_021987:c73988-73725 [Enterococcus faecium Aus0085]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT29897 NC_021987:c73988-73725 [Enterococcus faecium Aus0085]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A133CER3

Antitoxin

• AlphaFold DB: A0A133CED7