Detailed information of TA system TA029776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4634928..4635658
• Replicon: chromosome
• Accession: NC_021741
• Organism: Serratia liquefaciens ATCC 27592

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A515D591
• Locus tag: M495_RS21590
• Protein ID: WP_041415720.1
• Coordinates: 4634928..4635239 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M495_RS21595
• Protein ID: WP_020831880.1
• Coordinates: 4635236..4635658 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M495_RS21560	4630213..4630938	+	726	WP_020831867.1	LPS export ABC transporter ATP-binding protein	-
M495_RS21565	4630989..4632422	+	1434	WP_020831868.1	RNA polymerase factor sigma-54	-
M495_RS21570	4632446..4632733	+	288	WP_004948547.1	ribosome hibernation promoting factor	-
M495_RS21575	4632892..4633353	+	462	WP_099062098.1	PTS IIA-like nitrogen regulatory protein PtsN	-
M495_RS21580	4633485..4634339	+	855	WP_012147065.1	RNase adapter RapZ	-
M495_RS21585	4634336..4634608	+	273	WP_020831874.1	PTS phosphocarrier protein NPr	-
M495_RS21590	4634928..4635239	+	312	WP_041415720.1	type II toxin-antitoxin system HigB family toxin	Toxin
M495_RS21595	4635236..4635658	+	423	WP_020831880.1	helix-turn-helix domain-containing protein	Antitoxin
M495_RS21600	4635675..4637591	-	1917	WP_020831882.1	BglG family transcription antiterminator	-
M495_RS21605	4637643..4638860	-	1218	WP_020831883.1	lactonase family protein	-
M495_RS21610	4639012..4639752	-	741	WP_020831885.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12299.20 Da
• Isoelectric Point: 10.4493

>T29776 WP_041415720.1 NC_021741:4634928-4635239 [Serratia liquefaciens ATCC 27592]
MHIISREPFNEAAIRYPNEAASLNTIYKTLRRGTFHTPEDLKSLFPSLDRMKYKAKWWVIDVGGNHLRILFFASFDTQKI
FVKQIVTHAEYDRLLQQYRRNPK

Nucleotide

• Length: 312 bp

>T29776 NC_021741:4634928-4635239 [Serratia liquefaciens ATCC 27592]
ATGCATATAATTTCAAGGGAACCGTTCAACGAGGCCGCTATCCGCTACCCCAACGAAGCAGCCTCTCTCAACACAATCTA
CAAAACGCTGCGCCGAGGGACATTTCATACGCCGGAAGATTTAAAATCCCTTTTCCCCAGCCTGGACAGAATGAAGTACA
AAGCAAAATGGTGGGTCATTGATGTCGGAGGAAATCATCTAAGGATCTTATTCTTTGCCAGTTTTGATACCCAGAAAATA
TTTGTTAAACAGATAGTGACTCATGCAGAGTACGACAGATTACTGCAGCAGTACCGGAGGAACCCAAAATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15503.82 Da
• Isoelectric Point: 4.9381

>AT29776 WP_020831880.1 NC_021741:4635236-4635658 [Serratia liquefaciens ATCC 27592]
MITDALKATHELLRAIPLLGGSTSPQDYRDALALVEHLLETDERHPLIDVLAAKIVEYEDNSPDFAEFNQRIAQLPQGVA
ALSVLMEQYGLSQSDFENEIGKKSLVSQILSGKRSLTIPHIMALAKRFNLPPALFLPEAR

Nucleotide

• Length: 423 bp

>AT29776 NC_021741:4635236-4635658 [Serratia liquefaciens ATCC 27592]
ATGATCACCGATGCGCTTAAGGCCACCCATGAGCTGTTACGCGCCATACCCTTGTTAGGTGGCAGCACCTCGCCACAGGA
TTACCGTGATGCTCTGGCGTTAGTGGAACACCTGCTTGAAACCGACGAGCGCCACCCGCTGATTGACGTTCTGGCAGCCA
AGATCGTCGAGTATGAAGATAACAGCCCTGATTTTGCAGAGTTCAATCAGCGTATCGCACAGCTACCACAAGGTGTAGCC
GCGCTGAGCGTGCTGATGGAGCAATATGGCCTCAGTCAAAGCGACTTCGAAAACGAGATCGGCAAAAAGTCACTGGTGAG
CCAAATTCTAAGCGGAAAGCGATCGCTGACCATACCCCATATCATGGCGCTGGCAAAACGTTTCAACCTGCCGCCGGCCT
TGTTCTTGCCTGAAGCCCGTTAG

Structures

Toxin

• AlphaFold DB: A0A515D591