Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3536798..3537468 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag M943_RS16705 Protein ID WP_003899954.1
Coordinates 3536798..3537142 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag M943_RS16710 Protein ID WP_003899955.1
Coordinates 3537139..3537468 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS16675 3531871..3532731 + 861 WP_003416628.1 alpha/beta hydrolase -
M943_RS16680 3532706..3533221 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
M943_RS16685 3533461..3533754 + 294 WP_003416635.1 hypothetical protein -
M943_RS16690 3534042..3535331 + 1290 WP_003416640.1 ATP-binding protein -
M943_RS16695 3535678..3536112 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
M943_RS16700 3536115..3536567 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M943_RS16705 3536798..3537142 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M943_RS16710 3537139..3537468 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
M943_RS16715 3538006..3538353 + 348 WP_003899956.1 DUF2384 domain-containing protein -
M943_RS16720 3538350..3538970 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
M943_RS16725 3539130..3540395 - 1266 WP_003909837.1 hypothetical protein -
M943_RS16730 3540563..3540772 + 210 WP_003416778.1 hypothetical protein -
M943_RS16740 3541019..3542053 - 1035 WP_003905011.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T29755 WP_003899954.1 NC_021740:3536798-3537142 [Mycobacterium tuberculosis EAI5]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T29755 NC_021740:3536798-3537142 [Mycobacterium tuberculosis EAI5]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT29755 WP_003899955.1 NC_021740:3537139-3537468 [Mycobacterium tuberculosis EAI5]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT29755 NC_021740:3537139-3537468 [Mycobacterium tuberculosis EAI5]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References