Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2963016..2963664 Replicon chromosome
Accession NC_021740
Organism Mycobacterium tuberculosis EAI5

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag M943_RS13945 Protein ID WP_003899414.1
Coordinates 2963016..2963339 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag M943_RS13950 Protein ID WP_003899415.1
Coordinates 2963419..2963664 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M943_RS13915 2958336..2959334 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
M943_RS13920 2959348..2959812 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
M943_RS13925 2959800..2960054 + 255 WP_015631465.1 hypothetical protein -
M943_RS13930 2960225..2961664 - 1440 WP_009935349.1 phage major capsid protein -
M943_RS13935 2961672..2962205 - 534 WP_003899412.1 HK97 family phage prohead protease -
M943_RS21415 2962358..2962849 - 492 WP_003900541.1 phage terminase small subunit P27 family -
M943_RS13945 2963016..2963339 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
M943_RS13950 2963419..2963664 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
M943_RS13955 2963661..2965088 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
M943_RS13960 2965090..2965482 - 393 WP_003899417.1 DUF2742 domain-containing protein -
M943_RS13965 2965479..2965739 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
M943_RS13970 2965756..2966118 - 363 WP_003900543.1 hypothetical protein -
M943_RS13975 2966121..2967248 - 1128 WP_003899420.1 site-specific integrase -
M943_RS13980 2967393..2967620 - 228 WP_003899421.1 hypothetical protein -
M943_RS21420 2967617..2968006 - 390 WP_003899422.1 hypothetical protein -
M943_RS13985 2967912..2968184 + 273 WP_003900544.1 hypothetical protein -
M943_RS13990 2968283..2968516 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2958336..2967248 8912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T29747 WP_003899414.1 NC_021740:c2963339-2963016 [Mycobacterium tuberculosis EAI5]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T29747 NC_021740:c2963339-2963016 [Mycobacterium tuberculosis EAI5]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT29747 WP_003899415.1 NC_021740:c2963664-2963419 [Mycobacterium tuberculosis EAI5]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT29747 NC_021740:c2963664-2963419 [Mycobacterium tuberculosis EAI5]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References