Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4236695..4237374 Replicon chromosome
Accession NZ_OY019088
Organism Escherichia coli O25b:H4-ST131 isolate 42

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QU406_RS20530 Protein ID WP_000057523.1
Coordinates 4236695..4236997 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QU406_RS20535 Protein ID WP_000806442.1
Coordinates 4237033..4237374 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QU406_RS20505 (4232071) 4232071..4233723 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QU406_RS20510 (4233761) 4233761..4234264 - 504 WP_000667000.1 hypothetical protein -
QU406_RS20515 (4234261) 4234261..4235061 - 801 WP_000439798.1 hypothetical protein -
QU406_RS20520 (4235085) 4235085..4235564 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QU406_RS20525 (4235768) 4235768..4236562 - 795 WP_000365147.1 TraB/GumN family protein -
QU406_RS20530 (4236695) 4236695..4236997 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QU406_RS20535 (4237033) 4237033..4237374 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QU406_RS20540 (4237432) 4237432..4239936 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QU406_RS20545 (4240198) 4240198..4241130 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T297213 WP_000057523.1 NZ_OY019088:4236695-4236997 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT297213 WP_000806442.1 NZ_OY019088:4237033-4237374 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References