Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2077041..2077768 Replicon chromosome
Accession NZ_OY019088
Organism Escherichia coli O25b:H4-ST131 isolate 42

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QU406_RS10120 Protein ID WP_000550189.1
Coordinates 2077454..2077768 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QU406_RS10115 Protein ID WP_000560269.1
Coordinates 2077041..2077457 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QU406_RS10105 (2072401) 2072401..2074752 + 2352 WP_000695432.1 alpha-glucosidase -
QU406_RS10110 (2074978) 2074978..2076996 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QU406_RS10115 (2077041) 2077041..2077457 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QU406_RS10120 (2077454) 2077454..2077768 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QU406_RS10125 (2078053) 2078053..2079189 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QU406_RS10130 (2079274) 2079274..2079777 + 504 WP_001295542.1 M48 family metallopeptidase -
QU406_RS10135 (2079854) 2079854..2080546 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
QU406_RS10140 (2080625) 2080625..2081611 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T297202 WP_000550189.1 NZ_OY019088:c2077768-2077454 [Escherichia coli O25b:H4-ST131]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT297202 WP_000560269.1 NZ_OY019088:c2077457-2077041 [Escherichia coli O25b:H4-ST131]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References