Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3667101..3667780 Replicon chromosome
Accession NZ_OX637964
Organism Escherichia coli O25b:H4-ST131 isolate 46

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QQG90_RS18390 Protein ID WP_000057523.1
Coordinates 3667478..3667780 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQG90_RS18385 Protein ID WP_000806442.1
Coordinates 3667101..3667442 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQG90_RS18375 (3663345) 3663345..3664277 - 933 WP_000883041.1 glutaminase A -
QQG90_RS18380 (3664539) 3664539..3667043 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QQG90_RS18385 (3667101) 3667101..3667442 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQG90_RS18390 (3667478) 3667478..3667780 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQG90_RS18395 (3667913) 3667913..3668707 + 795 WP_000365147.1 TraB/GumN family protein -
QQG90_RS18400 (3668911) 3668911..3669390 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQG90_RS18405 (3669414) 3669414..3670214 + 801 WP_000439798.1 hypothetical protein -
QQG90_RS18410 (3670211) 3670211..3670714 + 504 WP_000667000.1 hypothetical protein -
QQG90_RS18415 (3670752) 3670752..3672404 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T297151 WP_000057523.1 NZ_OX637964:c3667780-3667478 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT297151 WP_000806442.1 NZ_OX637964:c3667442-3667101 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References