Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3567570..3568249 Replicon chromosome
Accession NZ_OX637961
Organism Escherichia coli O25b:H4-ST131 isolate 86

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QQG89_RS17480 Protein ID WP_000057523.1
Coordinates 3567947..3568249 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQG89_RS17475 Protein ID WP_000806442.1
Coordinates 3567570..3567911 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQG89_RS17465 (3563814) 3563814..3564746 - 933 WP_000883041.1 glutaminase A -
QQG89_RS17470 (3565008) 3565008..3567512 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
QQG89_RS17475 (3567570) 3567570..3567911 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQG89_RS17480 (3567947) 3567947..3568249 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQG89_RS17485 (3568382) 3568382..3569176 + 795 WP_000365147.1 TraB/GumN family protein -
QQG89_RS17490 (3569380) 3569380..3569859 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQG89_RS17495 (3569883) 3569883..3570683 + 801 WP_000439798.1 hypothetical protein -
QQG89_RS17500 (3570680) 3570680..3571183 + 504 WP_000667000.1 hypothetical protein -
QQG89_RS17505 (3571221) 3571221..3572873 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T297123 WP_000057523.1 NZ_OX637961:c3568249-3567947 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT297123 WP_000806442.1 NZ_OX637961:c3567911-3567570 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References