Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 38531..39282 Replicon plasmid EBP3064_p1
Accession NZ_OX461106
Organism Serratia proteamaculans strain EBP3064 isolate EBP3064

Toxin (Protein)


Gene name higB Uniprot ID A0A380D7R5
Locus tag QOT07_RS25395 Protein ID WP_017891116.1
Coordinates 38531..38842 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QOT07_RS25400 Protein ID WP_127147056.1
Coordinates 38839..39282 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QOT07_RS25375 33961..34467 + 507 WP_130016783.1 antirestriction protein -
QOT07_RS25380 35150..37186 + 2037 WP_130016782.1 ParB/RepB/Spo0J family partition protein -
QOT07_RS25385 37227..37691 + 465 WP_130016781.1 conjugation system SOS inhibitor PsiB -
QOT07_RS25390 37688..38416 + 729 WP_130016780.1 plasmid SOS inhibition protein A -
QOT07_RS25395 38531..38842 + 312 WP_017891116.1 type II toxin-antitoxin system HigB family toxin Toxin
QOT07_RS25400 38839..39282 + 444 WP_127147056.1 helix-turn-helix domain-containing protein Antitoxin
QOT07_RS25405 39846..40298 + 453 WP_129935340.1 hypothetical protein -
QOT07_RS25410 40359..40508 + 150 WP_165366321.1 hypothetical protein -
QOT07_RS25415 40955..41773 + 819 WP_130016779.1 DUF932 domain-containing protein -
QOT07_RS25420 42262..42603 - 342 WP_242503224.1 mobilization protein MobC -
QOT07_RS25425 42872..43177 + 306 WP_130016778.1 plasmid mobilization protein MobA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..108855 108855


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(92-146)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12245.20 Da        Isoelectric Point: 10.1438

>T297069 WP_017891116.1 NZ_OX461106:38531-38842 [Serratia proteamaculans]
VHVISRQPFNEAAGKYPNSALALLDLMRVLEKKTFNSPDEMKRAIPSLDNFKYRKKWWVIDVSGNTLRLMAFIDFEKQKV
FVKHIATHAEYDKLTKYYREHKE

Download         Length: 312 bp

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16064.33 Da        Isoelectric Point: 4.5904

>AT297069 WP_127147056.1 NZ_OX461106:38839-39282 [Serratia proteamaculans]
MTVATHIDAASAEGMIATFANAVKCIPLMTGDKSEAEYKKALELIEYLVDRDELSNPLFEPLAAKITEYENSAPEFEAFN
RRLAEIPTGVAALRTLMDQHGLKAADLEDELGSKSNVSNILSGRRALTVQHIKALATRFDVPADLFI

Download         Length: 444 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A380D7R5

Antitoxin

Source ID Structure

References