Detailed information of TA system TA296980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 66048..66641
• Replicon: chromosome
• Accession: NZ_OX458335
• Organism: Pseudomonas syringae pv. tomato strain DAPP-PG 215 isolate IPVPG 215

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: QWJ11_RS00320
• Protein ID: WP_007246530.1
• Coordinates: 66048..66326 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88B19
• Locus tag: QWJ11_RS00325
• Protein ID: WP_005739893.1
• Coordinates: 66336..66641 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QWJ11_RS00300 (DAPPPG215_00300)	61311..62549	-	1239	WP_007246526.1	diaminopimelate decarboxylase	-
QWJ11_RS00305 (DAPPPG215_00305)	62551..63426	-	876	WP_007246527.1	DMT family transporter	-
QWJ11_RS00310 (DAPPPG215_00310)	63426..64709	-	1284	WP_007246528.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
QWJ11_RS00315 (DAPPPG215_00315)	64864..65799	+	936	WP_007246529.1	LysR family transcriptional regulator	-
QWJ11_RS00320	66048..66326	+	279	WP_007246530.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QWJ11_RS00325 (DAPPPG215_00320)	66336..66641	+	306	WP_005739893.1	HigA family addiction module antitoxin	Antitoxin
QWJ11_RS00330 (DAPPPG215_00325)	66675..66956	-	282	WP_007246531.1	accessory factor UbiK family protein	-
QWJ11_RS00335 (DAPPPG215_00330)	67376..67714	+	339	WP_002555808.1	P-II family nitrogen regulator	-
QWJ11_RS00340 (DAPPPG215_00335)	67750..69087	+	1338	WP_005739890.1	ammonium transporter	-
QWJ11_RS00345 (DAPPPG215_00340)	69330..69755	+	426	WP_007246532.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
QWJ11_RS00350 (DAPPPG215_00345)	69854..70186	+	333	WP_007246533.1	transcriptional regulator SutA	-
QWJ11_RS00355 (DAPPPG215_00350)	70261..70953	-	693	WP_007246534.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10457.10 Da
• Isoelectric Point: 9.1490

>T296980 WP_007246530.1 NZ_OX458335:66048-66326 [Pseudomonas syringae pv. tomato]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEALDGSRSGQYSVRINAQFRICFVWSI
SGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T296980 NZ_OX458335:66048-66326 [Pseudomonas syringae pv. tomato]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGCTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGCGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AGCGGTCCCGAGGACGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11158.01 Da
• Isoelectric Point: 7.7509

>AT296980 WP_005739893.1 NZ_OX458335:66336-66641 [Pseudomonas syringae pv. tomato]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Nucleotide

• Length: 306 bp

>AT296980 NZ_OX458335:66336-66641 [Pseudomonas syringae pv. tomato]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGGGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8LYY6