Detailed information of TA system TA029684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3817..4468
• Replicon: plasmid pBJAB0715
• Accession: NC_021734
• Organism: Acinetobacter baumannii BJAB0715

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V2UF49
• Locus tag: BJAB0715_RS19255
• Protein ID: WP_000269909.1
• Coordinates: 3817..4173 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BJAB0715_RS19260
• Protein ID: WP_001140621.1
• Coordinates: 4166..4468 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJAB0715_RS19240	8..1033	-	1026	WP_079265503.1	IS30 family transposase	-
BJAB0715_RS19245	1113..1892	-	780	WP_000422636.1	aminoglycoside O-phosphotransferase APH(3')-VIa	-
BJAB0715_RS19250	1993..3018	-	1026	WP_079265503.1	IS30 family transposase	-
BJAB0715_RS19255	3817..4173	+	357	WP_000269909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJAB0715_RS19260	4166..4468	+	303	WP_001140621.1	XRE family transcriptional regulator	Antitoxin
BJAB0715_RS19265	4461..4670	+	210	WP_000069474.1	hypothetical protein	-
BJAB0715_RS19270	4845..5363	+	519	WP_000447196.1	sel1 repeat family protein	-
BJAB0715_RS19275	5402..5914	+	513	WP_001089572.1	SMI1/KNR4 family protein	-
BJAB0715_RS19280	6157..7080	+	924	WP_020753572.1	hypothetical protein	-
BJAB0715_RS19285	7291..7857	-	567	WP_000678311.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / cmlB1 / aac(3)-IId / blaOXA-58	-	1..52268	52268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13515.49 Da
• Isoelectric Point: 6.4631

>T29684 WP_000269909.1 NC_021734:3817-4173 [Acinetobacter baumannii BJAB0715]
MWTVITTDLFNEWLVQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T29684 NC_021734:3817-4173 [Acinetobacter baumannii BJAB0715]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGTACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ACATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAACT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11179.02 Da
• Isoelectric Point: 7.3198

>AT29684 WP_001140621.1 NC_021734:4166-4468 [Acinetobacter baumannii BJAB0715]
MAKTLQELLASRSPESQARIQKMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT29684 NC_021734:4166-4468 [Acinetobacter baumannii BJAB0715]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTGGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Structures

Toxin

• AlphaFold DB: V2UF49