Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 5295226..5295915 Replicon chromosome
Accession NZ_OX359182
Organism Klebsiella pneumoniae strain KP34 isolate 2018-347802-001-01

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag OM989_RS25440 Protein ID WP_021469727.1
Coordinates 5295598..5295915 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag OM989_RS25435 Protein ID WP_020804705.1
Coordinates 5295226..5295522 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM989_RS25420 (5291618) 5291618..5293000 + 1383 WP_004151222.1 MFS transporter -
OM989_RS25425 (5293044) 5293044..5294660 + 1617 WP_004175961.1 carbohydrate porin -
OM989_RS25430 (5294701) 5294701..5295147 - 447 WP_032435212.1 hypothetical protein -
OM989_RS25435 (5295226) 5295226..5295522 - 297 WP_020804705.1 NadS family protein Antitoxin
OM989_RS25440 (5295598) 5295598..5295915 - 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OM989_RS25445 (5296122) 5296122..5296349 - 228 WP_002906690.1 tautomerase PptA -
OM989_RS25450 (5296420) 5296420..5297037 - 618 WP_032425015.1 glutathione S-transferase family protein -
OM989_RS25455 (5297115) 5297115..5297966 - 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
OM989_RS25460 (5298005) 5298005..5298784 - 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
OM989_RS25465 (5298768) 5298768..5299694 - 927 WP_032435210.1 amino acid ABC transporter permease -
OM989_RS25470 (5299704) 5299704..5300213 - 510 WP_020802144.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T296727 WP_021469727.1 NZ_OX359182:c5295915-5295598 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT296727 WP_020804705.1 NZ_OX359182:c5295522-5295226 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References