Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2514595..2515284 Replicon chromosome
Accession NZ_OX359175
Organism Klebsiella pneumoniae strain KP47 isolate 2018-047100-007-01

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag ONI05_RS12325 Protein ID WP_021469727.1
Coordinates 2514595..2514912 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag ONI05_RS12330 Protein ID WP_020804705.1
Coordinates 2514988..2515284 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ONI05_RS12295 (2510297) 2510297..2510806 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
ONI05_RS12300 (2510816) 2510816..2511742 + 927 WP_032435210.1 amino acid ABC transporter permease -
ONI05_RS12305 (2511726) 2511726..2512505 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
ONI05_RS12310 (2512544) 2512544..2513395 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
ONI05_RS12315 (2513473) 2513473..2514090 + 618 WP_032425015.1 glutathione S-transferase family protein -
ONI05_RS12320 (2514161) 2514161..2514388 + 228 WP_002906690.1 tautomerase PptA -
ONI05_RS12325 (2514595) 2514595..2514912 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ONI05_RS12330 (2514988) 2514988..2515284 + 297 WP_020804705.1 NadS family protein Antitoxin
ONI05_RS12335 (2515363) 2515363..2515809 + 447 WP_032435212.1 hypothetical protein -
ONI05_RS12340 (2515850) 2515850..2517466 - 1617 WP_004175961.1 carbohydrate porin -
ONI05_RS12345 (2517510) 2517510..2518892 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T296700 WP_021469727.1 NZ_OX359175:2514595-2514912 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT296700 WP_020804705.1 NZ_OX359175:2514988-2515284 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References