296558

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4526125..4526819 Replicon chromosome
Accession NZ_OX030728
Organism Escherichia coli isolate 68

Toxin (Protein)


Gene name yafO Uniprot ID V0YKG6
Locus tag LQ200_RS21695 Protein ID WP_001263485.1
Coordinates 4526421..4526819 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LQ200_RS21690 Protein ID WP_000554758.1
Coordinates 4526125..4526418 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ200_RS21670 (4521631) 4521631..4522380 + 750 WP_000093945.1 C40 family peptidase -
LQ200_RS21675 (4522478) 4522478..4524190 - 1713 Protein_4235 flagellar biosynthesis protein FlhA -
LQ200_RS21680 (4524162) 4524162..4524947 + 786 WP_000207541.1 putative lateral flagellar export/assembly protein LafU -
LQ200_RS21685 (4525018) 4525018..4526073 + 1056 WP_001226177.1 DNA polymerase IV -
LQ200_RS21690 (4526125) 4526125..4526418 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LQ200_RS21695 (4526421) 4526421..4526819 + 399 WP_001263485.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LQ200_RS21700 (4526829) 4526829..4527281 + 453 WP_001059900.1 GNAT family N-acetyltransferase -
LQ200_RS21705 (4527600) 4527600..4528739 + 1140 WP_000521554.1 RNA ligase RtcB family protein -
LQ200_RS21710 (4528736) 4528736..4529350 + 615 WP_000602129.1 peptide chain release factor H -
LQ200_RS21715 (4529412) 4529412..4529921 + 510 WP_000469793.1 metal-dependent hydrolase -
LQ200_RS21720 (4529938) 4529938..4530219 - 282 WP_000106438.1 hypothetical protein -
LQ200_RS21725 (4530228) 4530228..4531685 - 1458 WP_001293011.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15418.84 Da        Isoelectric Point: 7.3840

>T296558 WP_001263485.1 NZ_OX030728:4526421-4526819 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT296558 WP_000554758.1 NZ_OX030728:4526125-4526418 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YKG6

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References