Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 325546..326212 Replicon chromosome
Accession NZ_OW969904
Organism Citrobacter freundii isolate 0

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag LQ142_RS01515 Protein ID WP_003847996.1
Coordinates 325895..326212 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag LQ142_RS01510 Protein ID WP_003837894.1
Coordinates 325546..325842 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ142_RS01495 (322722) 322722..323195 - 474 WP_003023524.1 transcription elongation factor GreB -
LQ142_RS01500 (323421) 323421..324140 + 720 WP_001157751.1 two-component system response regulator OmpR -
LQ142_RS01505 (324137) 324137..325489 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
LQ142_RS01510 (325546) 325546..325842 - 297 WP_003837894.1 NadS family protein Antitoxin
LQ142_RS01515 (325895) 325895..326212 - 318 WP_003847996.1 hypothetical protein Toxin
LQ142_RS01520 (326335) 326335..327957 - 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
LQ142_RS01525 (328336) 328336..330054 + 1719 WP_047715944.1 DUF4153 domain-containing protein -
LQ142_RS01530 (330164) 330164..331042 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T296194 WP_003847996.1 NZ_OW969904:c326212-325895 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT296194 WP_003837894.1 NZ_OW969904:c325842-325546 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References