Detailed information of TA system TA296180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4415853..4416580
• Replicon: chromosome
• Accession: NZ_OW969711
• Organism: Citrobacter koseri isolate 0

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LQV24_RS20935
• Protein ID: WP_047459826.1
• Coordinates: 4416272..4416580 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LQV24_RS20930
• Protein ID: WP_199972370.1
• Coordinates: 4415853..4416272 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQV24_RS20905 (4411049)	4411049..4411978	+	930	WP_047459819.1	1,4-dihydroxy-2-naphthoate polyprenyltransferase	-
LQV24_RS20910 (4412071)	4412071..4412556	+	486	WP_012133864.1	ribonuclease E activity regulator RraA	-
LQV24_RS20915 (4412722)	4412722..4412967	-	246	WP_012133867.1	septal ring assembly protein ZapB	-
LQV24_RS20920 (4413358)	4413358..4414203	+	846	WP_012133869.1	MIP/aquaporin family protein	-
LQV24_RS20925 (4414227)	4414227..4415735	+	1509	WP_012133870.1	glycerol kinase GlpK	-
LQV24_RS20930 (4415853)	4415853..4416272	-	420	WP_199972370.1	helix-turn-helix domain-containing protein	Antitoxin
LQV24_RS20935 (4416272)	4416272..4416580	-	309	WP_047459826.1	type II toxin-antitoxin system HigB family toxin	Toxin
LQV24_RS20940 (4416721)	4416721..4417731	+	1011	WP_012133873.1	class II fructose-bisphosphatase	-
LQV24_RS20945 (4417827)	4417827..4418573	+	747	WP_012133874.1	ferredoxin--NADP(+) reductase	-
LQV24_RS20950 (4418643)	4418643..4419089	-	447	WP_049011788.1	DUF805 domain-containing protein	-
LQV24_RS20955 (4419190)	4419190..4419786	+	597	WP_047459831.1	YiiQ family protein	-
LQV24_RS20960 (4419901)	4419901..4420668	+	768	WP_024130662.1	triose-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 12352.15 Da
• Isoelectric Point: 10.0486

>T296180 WP_047459826.1 NZ_OW969711:c4416580-4416272 [Citrobacter koseri]
MHVISKEPFEEAAKRYPNDSLAIRALYRLMRETDFSSPTEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHISTHAEYDKLTRYYRENK

Nucleotide

• Length: 309 bp

>T296180 NZ_OW969711:c4416580-4416272 [Citrobacter koseri]
ATGCACGTCATTTCAAAAGAACCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATACGGGCGCTGTA
CCGCTTAATGCGTGAAACTGACTTCTCTTCTCCGACTGAGATGAGAACTCTGATACCGAGCCTGGACAATTTTAAGTACC
GGAACAAATGGTGGGTTCTGGATGTGGGAGGCAACAACTTGAGGGTTATCGCCTACATCAATTTTGTGAACAAACGCTTC
TATGTGAAGCACATCAGCACCCACGCTGAATATGACAAGTTGACCCGCTATTACAGGGAGAACAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15350.35 Da
• Isoelectric Point: 4.3485

>AT296180 WP_199972370.1 NZ_OW969711:c4416272-4415853 [Citrobacter koseri]
MNTDTAKAIEATKQLVAAVPFLGGSSSESDYREALSLVDYLIENDDENPLIDFLASKIADYEDNSDRFAEFNKGIAEMPV
GVALLRTLIDQYKLSYSDLKEEIGSKSLVSQILSGQRSLTIAHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT296180 NZ_OW969711:c4416272-4415853 [Citrobacter koseri]
ATGAATACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTCGCCGCTGTTCCTTTCCTGGGAGGCAGCTCTTC
AGAGAGCGATTACCGTGAAGCGCTGTCTCTGGTGGATTATCTGATCGAGAACGACGATGAGAACCCGCTCATTGACTTCC
TGGCGAGCAAAATTGCCGACTATGAGGATAACAGCGACCGTTTCGCTGAGTTCAATAAAGGTATCGCTGAAATGCCGGTT
GGCGTGGCGCTGCTACGTACTCTGATTGACCAGTACAAGCTGTCCTACTCAGACCTGAAAGAGGAGATCGGTTCAAAGTC
GCTGGTGAGTCAGATCTTATCTGGTCAGCGCTCTCTGACTATCGCTCACATTAAGGCACTCTCTGCTCGCTTCGGTGTGA
AGCCAGAGTGGTTTCTTTAA