296072

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3785720..3786414 Replicon chromosome
Accession NZ_OW968288
Organism Escherichia coli isolate 10

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LQ134_RS18445 Protein ID WP_001263489.1
Coordinates 3785720..3786118 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LQ134_RS18450 Protein ID WP_000554758.1
Coordinates 3786121..3786414 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3781308) 3781308..3781388 - 81 NuclAT_11 - -
- (3781308) 3781308..3781388 - 81 NuclAT_11 - -
- (3781308) 3781308..3781388 - 81 NuclAT_11 - -
- (3781308) 3781308..3781388 - 81 NuclAT_11 - -
LQ134_RS18420 (3781984) 3781984..3782442 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LQ134_RS18425 (3782703) 3782703..3784160 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LQ134_RS18430 (3784217) 3784217..3784738 - 522 Protein_3627 peptide chain release factor H -
LQ134_RS18435 (3784734) 3784734..3784940 - 207 Protein_3628 RtcB family protein -
LQ134_RS18440 (3785258) 3785258..3785710 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
LQ134_RS18445 (3785720) 3785720..3786118 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LQ134_RS18450 (3786121) 3786121..3786414 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LQ134_RS18455 (3786466) 3786466..3787521 - 1056 WP_001226164.1 DNA polymerase IV -
LQ134_RS18460 (3787592) 3787592..3788377 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LQ134_RS18465 (3788349) 3788349..3790061 + 1713 Protein_3634 flagellar biosynthesis protein FlhA -
LQ134_RS18470 (3790285) 3790285..3790782 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T296072 WP_001263489.1 NZ_OW968288:c3786118-3785720 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT296072 WP_000554758.1 NZ_OW968288:c3786414-3786121 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References