Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3761090..3761784 Replicon chromosome
Accession NZ_OW968124
Organism Escherichia coli isolate 10

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LQ159_RS18325 Protein ID WP_001263489.1
Coordinates 3761090..3761488 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LQ159_RS18330 Protein ID WP_000554758.1
Coordinates 3761491..3761784 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3756678) 3756678..3756758 - 81 NuclAT_11 - -
- (3756678) 3756678..3756758 - 81 NuclAT_11 - -
- (3756678) 3756678..3756758 - 81 NuclAT_11 - -
- (3756678) 3756678..3756758 - 81 NuclAT_11 - -
LQ159_RS18300 (3757354) 3757354..3757812 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LQ159_RS18305 (3758073) 3758073..3759530 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LQ159_RS18310 (3759587) 3759587..3760108 - 522 Protein_3602 peptide chain release factor H -
LQ159_RS18315 (3760104) 3760104..3760310 - 207 Protein_3603 RtcB family protein -
LQ159_RS18320 (3760628) 3760628..3761080 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
LQ159_RS18325 (3761090) 3761090..3761488 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LQ159_RS18330 (3761491) 3761491..3761784 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LQ159_RS18335 (3761836) 3761836..3762891 - 1056 WP_001226164.1 DNA polymerase IV -
LQ159_RS18340 (3762962) 3762962..3763747 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LQ159_RS18345 (3763719) 3763719..3765431 + 1713 Protein_3609 flagellar biosynthesis protein FlhA -
LQ159_RS18350 (3765655) 3765655..3766152 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3761090..3776370 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T296000 WP_001263489.1 NZ_OW968124:c3761488-3761090 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT296000 WP_000554758.1 NZ_OW968124:c3761784-3761491 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References