Detailed information of TA system TA029599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4666184..4666935
• Replicon: chromosome
• Accession: NC_021591
• Organism: Serratia plymuthica 4Rx13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A318P873
• Locus tag: SOD_RS21165
• Protein ID: WP_004948411.1
• Coordinates: 4666184..4666495 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SOD_RS21170
• Protein ID: WP_172458758.1
• Coordinates: 4666534..4666935 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SOD_RS26055	4662930..4663337	+	408	WP_172458755.1	hypothetical protein	-
SOD_RS21150	4663382..4663888	+	507	WP_004948406.1	antirestriction protein	-
SOD_RS21155	4664114..4665184	+	1071	WP_004948409.1	colicin B	-
SOD_RS21160	4665240..4665752	-	513	WP_041417191.1	colicin immunity protein Cui	-
SOD_RS21165	4666184..4666495	+	312	WP_004948411.1	type II toxin-antitoxin system HigB family toxin	Toxin
SOD_RS21170	4666534..4666935	+	402	WP_172458758.1	helix-turn-helix domain-containing protein	Antitoxin
SOD_RS25910	4667062..4667124	-	63	Protein_4199	YgjV family protein	-
SOD_RS21175	4667196..4668686	-	1491	WP_004948416.1	altronate dehydratase family protein	-
SOD_RS21180	4668707..4670119	-	1413	WP_004948418.1	glucuronate isomerase	-
SOD_RS21185	4670505..4671803	+	1299	WP_004948421.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12272.23 Da
• Isoelectric Point: 10.1438

>T29599 WP_004948411.1 NC_021591:4666184-4666495 [Serratia plymuthica 4Rx13]
VHVINRQPFNEAAGKYPNSALALLDLMRVLEKKTFNSPDEMKRAIPSLDNFKYRKKWWVIDVSGNTLRLMAFIDFEKQKV
FVKHIATHAEYDKLTKYYREHKE

Nucleotide

• Length: 312 bp

>T29599 NC_021591:4666184-4666495 [Serratia plymuthica 4Rx13]
GTGCATGTAATTAACAGACAGCCGTTTAACGAGGCTGCCGGAAAATACCCTAATAGCGCATTGGCGCTTCTGGATTTGAT
GCGGGTTCTGGAAAAAAAGACCTTCAACAGCCCGGATGAAATGAAAAGAGCGATCCCGTCACTGGATAACTTCAAGTACC
GTAAAAAGTGGTGGGTTATCGATGTGTCCGGTAATACGTTGCGGCTTATGGCCTTCATCGACTTTGAAAAGCAAAAGGTT
TTTGTTAAGCACATTGCGACTCATGCCGAATACGACAAGTTAACCAAGTATTATAGGGAGCATAAAGAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14738.87 Da
• Isoelectric Point: 4.6655

>AT29599 WP_172458758.1 NC_021591:4666534-4666935 [Serratia plymuthica 4Rx13]
MIATFANAVKCIPLMTGDKSETEYKKALELIEYLVDRDELSNPLFEPLAAKITEYENSAPEFEAFNRRLAEIPTGVAALR
TLMDQHGLKAADLEDELGSKSNVSNILSGRRALTVQHIKALATRFDVPADLFI

Nucleotide

• Length: 402 bp

>AT29599 NC_021591:4666534-4666935 [Serratia plymuthica 4Rx13]
ATGATCGCGACATTTGCCAACGCGGTGAAATGCATTCCTCTGATGACGGGTGATAAAAGCGAGACTGAATACAAAAAAGC
CCTTGAGCTGATTGAGTATCTTGTCGATCGCGACGAACTGAGCAACCCGCTTTTTGAACCGCTTGCGGCCAAAATTACAG
AGTATGAAAACAGCGCCCCGGAATTTGAGGCGTTTAACCGCCGCTTGGCGGAGATCCCTACCGGGGTCGCCGCGTTGCGT
ACCCTGATGGATCAGCATGGTTTGAAAGCGGCTGATCTGGAAGATGAATTGGGGTCTAAAAGTAATGTCAGTAACATTCT
CAGTGGTCGCCGTGCGTTGACTGTGCAGCACATTAAGGCGCTGGCGACGCGTTTTGATGTCCCTGCTGATTTGTTTATTT
GA

Structures

Toxin

• AlphaFold DB: A0A318P873