Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4002871..4003412 Replicon chromosome
Accession NZ_OW968011
Organism Escherichia coli isolate 58

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LQ149_RS19095 Protein ID WP_000615976.1
Coordinates 4002871..4003149 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LQ149_RS19100 Protein ID WP_000729704.1
Coordinates 4003152..4003412 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ149_RS19080 (4000453) 4000453..4001031 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
LQ149_RS19085 (4001237) 4001237..4002004 + 768 WP_000333380.1 class II glutamine amidotransferase -
LQ149_RS19090 (4001975) 4001975..4002715 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LQ149_RS19095 (4002871) 4002871..4003149 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LQ149_RS19100 (4003152) 4003152..4003412 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LQ149_RS19105 (4003598) 4003598..4004371 + 774 WP_000543895.1 C40 family peptidase -
LQ149_RS19110 (4004428) 4004428..4004784 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
LQ149_RS19115 (4004777) 4004777..4005055 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LQ149_RS19120 (4005160) 4005160..4006872 - 1713 Protein_3730 flagellar biosynthesis protein FlhA -
LQ149_RS19125 (4006844) 4006844..4007629 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 3987456..4005055 17599


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T295957 WP_000615976.1 NZ_OW968011:c4003149-4002871 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT295957 WP_000729704.1 NZ_OW968011:c4003412-4003152 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References