Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3753222..3753916 Replicon chromosome
Accession NZ_OW967975
Organism Escherichia coli isolate 10

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LQ150_RS18260 Protein ID WP_001263489.1
Coordinates 3753222..3753620 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LQ150_RS18265 Protein ID WP_000554758.1
Coordinates 3753623..3753916 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3748810) 3748810..3748890 - 81 NuclAT_11 - -
- (3748810) 3748810..3748890 - 81 NuclAT_11 - -
- (3748810) 3748810..3748890 - 81 NuclAT_11 - -
- (3748810) 3748810..3748890 - 81 NuclAT_11 - -
LQ150_RS18235 (3749486) 3749486..3749944 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LQ150_RS18240 (3750205) 3750205..3751662 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LQ150_RS18245 (3751719) 3751719..3752240 - 522 Protein_3589 peptide chain release factor H -
LQ150_RS18250 (3752236) 3752236..3752442 - 207 Protein_3590 RtcB family protein -
LQ150_RS18255 (3752760) 3752760..3753212 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
LQ150_RS18260 (3753222) 3753222..3753620 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LQ150_RS18265 (3753623) 3753623..3753916 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LQ150_RS18270 (3753968) 3753968..3755023 - 1056 WP_001226164.1 DNA polymerase IV -
LQ150_RS18275 (3755094) 3755094..3755879 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LQ150_RS18280 (3755851) 3755851..3757563 + 1713 Protein_3596 flagellar biosynthesis protein FlhA -
LQ150_RS18285 (3757787) 3757787..3758284 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3753222..3768502 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T295915 WP_001263489.1 NZ_OW967975:c3753620-3753222 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT295915 WP_000554758.1 NZ_OW967975:c3753916-3753623 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References