Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1111655..1112382 Replicon chromosome
Accession NZ_OW967802
Organism Escherichia coli isolate 410

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag LQ147_RS05420 Protein ID WP_000547564.1
Coordinates 1111655..1111966 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LQ147_RS05425 Protein ID WP_000126294.1
Coordinates 1111963..1112382 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ147_RS05390 (1106797) 1106797..1108506 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
LQ147_RS05395 (1108516) 1108516..1109058 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
LQ147_RS05400 (1109058) 1109058..1109825 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
LQ147_RS05405 (1109822) 1109822..1110232 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
LQ147_RS05410 (1110225) 1110225..1110695 + 471 WP_060621146.1 hydrogenase maturation peptidase HycI -
LQ147_RS05415 (1110720) 1110720..1111481 + 762 WP_001026446.1 hypothetical protein -
LQ147_RS05420 (1111655) 1111655..1111966 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
LQ147_RS05425 (1111963) 1111963..1112382 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
LQ147_RS05430 (1112496) 1112496..1113920 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
LQ147_RS05435 (1113929) 1113929..1115386 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
LQ147_RS05440 (1115646) 1115646..1116656 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
LQ147_RS05445 (1116805) 1116805..1117332 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T295857 WP_000547564.1 NZ_OW967802:1111655-1111966 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT295857 WP_000126294.1 NZ_OW967802:1111963-1112382 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References